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Paper · 19633652

Programming cells by multiplex genome engineering and accelerated evolution.

Wang HH and 6 others. Nature, 2009. Cited by 1,142 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Programming cells by multiplex genome engineering and accelerated evolution. Papers, record 19633652. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/19633652

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
Programming cells by multiplex genome engineering and accelerated evolution.
PubMed
Journal
Nature
PubMed
Year
2009
PubMed
Publication types
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
Citations, by iCite
1,142
iCite
Relative citation ratio, by iCite
27.6
iCite
Revised in PubMed
2024-01-04
PubMed

Authors7

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Wang HHHarris H
2Isaacs FJFarren J
3Carr PAPeter A
4Sun ZZZachary Z
5Xu GGeorge
6Forest CRCraig R
7Church GMGeorge M

Source PubMed

Topics11

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
BiotechnologyMajor topic
Directed Molecular EvolutionMajor topic
Escherichia coliMajor topic
Genome, BacterialMajor topic
Alleles
Carotenoids
Chromosomes, Bacterial
DNA
Genetic Variation
Lycopene
Pentosephosphates

Source PubMed

Grants1

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated by
DP5 OD009172NIH HHSUnited StatesDP5OD009172PubMed and NIH

Source NIH RePORTER

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References32

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
115,616,567Accurate multiplex gene synthesis from programmable DNA microchips.Nature2004https://conductscience.org/library/papers/15616567PubMed
215,001,713Environmental genome shotgun sequencing of the Sargasso Sea.Science2004https://conductscience.org/library/papers/15001713PubMed
318,230,724Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages.Proc Natl Acad Sci U S A2008https://conductscience.org/library/papers/18230724PubMed
411,180,061Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production.Biotechnol Bioeng2001https://conductscience.org/library/papers/11180061PubMed
518,218,864Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.Science2008https://conductscience.org/library/papers/18218864PubMed
615,821,729Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets.Nat Biotechnol2005https://conductscience.org/library/papers/15821729PubMed
715,522,458An expanding genetic code.Trends Genet2004https://conductscience.org/library/papers/15522458PubMed
812,776,215Evolution experiments with microorganisms: the dynamics and genetic bases of adaptation.Nat Rev Genet2003https://conductscience.org/library/papers/12776215PubMed
914,673,109Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.Proc Natl Acad Sci U S A2003https://conductscience.org/library/papers/14673109PubMed
1010,385,118Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation.Nature1999https://conductscience.org/library/papers/10385118PubMed

1 to 10 of 32

See all 32 references

Cited by687

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 1,142 citations.

PMIDTitleJournalYearIn PapersSource
42,961,742Genome-Scale Metabolic Modeling of Escherichia coli and Its Chassis Design for Synthetic Biology Applications.Methods Mol Biol2027https://conductscience.org/library/papers/42961742PubMed
42,959,715Design and artificial synthesis of gRNA library for genetic breeding of Saccharomyces cerevisiae.Biodes Res2026https://conductscience.org/library/papers/42959715PubMed
42,891,351Strategies and mechanisms of precision genome engineering: From gene editing to genome writing.IMetaOmics2026https://conductscience.org/library/papers/42891351PubMed
42,844,390Predicting resistance evolution to guide antibiotic development.Nat Rev Microbiol2026https://conductscience.org/library/papers/42844390PubMed
42,844,378Retention and biosynthesis of diverse nitroarenes in Escherichia coli after combinatorial nitroreductase gene deletions.Nat Chem2026https://conductscience.org/library/papers/42844378PubMed
42,661,411Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression.Biotechnol J2026https://conductscience.org/library/papers/42661411PubMed
42,588,511Challenges and Technological Strategies to Enhance Probiotic Viability in Non-Dairy Food Matrices.Molecules2026https://conductscience.org/library/papers/42588511PubMed
42,573,069A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli.Nucleic Acids Res2026https://conductscience.org/library/papers/42573069PubMed
42,464,282Establishment of SRLC: a multiplex genome editing technology for Saccharomyces cerevisiae and its application in metabolic engineering of malonyl-CoA pathway.Microb Cell Fact2026https://conductscience.org/library/papers/42464282PubMed
42,405,764EndoV does not measurably affect TadA-dependent A-to-I RNA editing in Escherichia coli under exponential-growth conditions in rich medium.Microbiol Spectr2026https://conductscience.org/library/papers/42405764PubMed

1 to 10 of 687

See all 687 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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