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Paper · 242001

Specificity of substrate recognition by the EcoRI restriction endonuclease.

Polisky B and 5 others. Proc Natl Acad Sci U S A, 1975. Cited by 337 papers, by iCite.

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ConductScience Library. Specificity of substrate recognition by the EcoRI restriction endonuclease. Papers, record 242001. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/242001

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
Specificity of substrate recognition by the EcoRI restriction endonuclease.
PubMed
Journal
Proc Natl Acad Sci U S A
PubMed
Year
1975
PubMed
Publication types
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
PubMed
Citations, by iCite
337
iCite
Relative citation ratio, by iCite
8.22
iCite
Revised in PubMed
2019-05-01
PubMed

Authors6

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Polisky BB
2Greene PP
3Garfin DED E
4McCarthy BJB J
5Goodman HMH M
6Boyer HWH W

Source PubMed

Topics9

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
DNA Restriction EnzymesMajor topic
EndonucleasesMajor topic
Escherichia coliMajor topic
Base Sequence
Binding Sites
DNA
Hydrogen-Ion Concentration
Osmolar Concentration
Structure-Activity Relationship

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References20

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
14,610,576Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.Proc Natl Acad Sci U S A1974https://conductscience.org/library/papers/4610576PubMed
24,358,949Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase.Biochemistry1974https://conductscience.org/library/papers/4358949PubMed
34,355,584Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli.J Biol Chem1973https://conductscience.org/library/papers/4355584PubMed
44,365,585Polyoma DNA: a physical map.Proc Natl Acad Sci U S A1974https://conductscience.org/library/papers/4365585PubMed
54,366,956Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.Biochem Biophys Res Commun1974https://conductscience.org/library/papers/4366956PubMed
64,372,623Sequence arrangements in clonal isolates of polyoma defective DNA.Proc Natl Acad Sci U S A1974https://conductscience.org/library/papers/4372623PubMed
74,372,397Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.J Virol1974https://conductscience.org/library/papers/4372397PubMed
84,334,983Purification of the DNA polymerase of avian myeloblastosis virus.Biochim Biophys Acta1971https://conductscience.org/library/papers/4334983PubMed
94,343,974DNA nucleotide sequence restricted by the RI endonuclease.Proc Natl Acad Sci U S A1972https://conductscience.org/library/papers/4343974PubMed
104,343,967Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme.Proc Natl Acad Sci U S A1972https://conductscience.org/library/papers/4343967PubMed

1 to 10 of 20

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Cited by121

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 337 citations.

PMIDTitleJournalYearIn PapersSource
41,059,593Promoting biotechnology applications in China through effective biosafety education and communication.GM Crops Food2025https://conductscience.org/library/papers/41059593PubMed
40,547,456INNSE: Invertible neural network-based DNA image storage with self-correction encoding.Comput Struct Biotechnol J2025https://conductscience.org/library/papers/40547456PubMed
39,073,829Explorer: efficient DNA coding by De Bruijn graph toward arbitrary local and global biochemical constraints.Brief Bioinform2024https://conductscience.org/library/papers/39073829PubMed
38,461,245DNA-QLC: an efficient and reliable image encoding scheme for DNA storage.BMC Genomics2024https://conductscience.org/library/papers/38461245PubMed
38,262,299Evaluation of restriction and Cas endonuclease kinetics using matrix-insensitive magnetic biosensors.Biosens Bioelectron2024https://conductscience.org/library/papers/38262299PubMed
37,974,977Protein-DNA recognition mechanisms and specificity.Biophys Rev2023https://conductscience.org/library/papers/37974977PubMed
34,680,052Kinetic Analysis of the Interaction of Nicking Endonuclease BspD6I with DNA.Biomolecules2021https://conductscience.org/library/papers/34680052PubMed
29,789,800LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity.Biomed Res Int2018https://conductscience.org/library/papers/29789800PubMed
25,567,985Multilayered genetic safeguards limit growth of microorganisms to defined environments.Nucleic Acids Res2015https://conductscience.org/library/papers/25567985PubMed
24,878,924Type II restriction endonucleases--a historical perspective and more.Nucleic Acids Res2014https://conductscience.org/library/papers/24878924PubMed

1 to 10 of 121

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Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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