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Paper · 2744487

Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Ho SN and 4 others. Gene, 1989. Cited by 7,226 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Papers, record 2744487. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/2744487

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
PubMed
Journal
Gene
PubMed
Year
1989
PubMed
Publication types
Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
PubMed
Citations, by iCite
7,226
iCite
Relative citation ratio, by iCite
159
iCite
Revised in PubMed
2024-01-09
PubMed

Authors5

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Ho SNS N
2Hunt HDH D
3Horton RMR M
4Pullen JKJ K
5Pease LRL R

Source PubMed

Topics14

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
Genes, MHC Class IMajor topic
Genes, SyntheticMajor topic
Genetic EngineeringMajor topic
MutationMajor topic
Amino Acid Sequence
Animals
DNA, Recombinant
DNA-Directed DNA Polymerase
Exons
Gene Amplification
Mice
Molecular Sequence Data
Oligodeoxyribonucleotides
Taq Polymerase

Source PubMed

Grants3

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated bySource
AI-00706NIAID NIH HHSUnited StatesPubMedPubMed
AI-22420NIAID NIH HHSUnited StatesR01AI022420PubMed and NIHNIH RePORTER
CA-09127NCI NIH HHSUnited StatesPubMedPubMed

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References0

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

None in this release.

Cited by2,940

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 7,226 citations.

PMIDTitleJournalYearIn PapersSource
42,800,801Integrative structural modelling reveals the human Mic60-Mic19 subcomplex as a diffusion barrier in mitochondria.Nat Commun2026https://conductscience.org/library/papers/42800801PubMed
42,734,791Molecular Cloning and Reverse Genetics.Methods Mol Biol2027https://conductscience.org/library/papers/42734791PubMed
42,653,503Molecular Dissection of the SlBAG9 Promoter from Tomato and Its Thermo-Regulatory Activity.Int J Mol Sci2026https://conductscience.org/library/papers/42653503PubMed
42,557,407A mechanistic framework for the recognition of chemically diverse brassinosteroids by BRI1-family receptor kinases.Nat Plants2026https://conductscience.org/library/papers/42557407PubMed
42,496,281Aglycosylated Immunoglobulin G1 Fc Stabilized Through Disulfide Bond Addition Exhibits Compositional Homogeneity and Retains Fc γ Receptor IIIa/CD16a Binding.Antibodies (Basel)2026https://conductscience.org/library/papers/42496281PubMed
42,473,763A proximity ligation screen identifies SNAT2 as a novel target of the MARCH1 E3 ubiquitin ligase.Biol Open2026https://conductscience.org/library/papers/42473763PubMed
42,438,306A Nutrient-Responsive LuxR Regulator Orchestrates Effector Gene Expression Across the Legionella Genus.Mol Microbiol2026https://conductscience.org/library/papers/42438306PubMed
42,420,857Elucidating the biochemical properties of limonene synthase from Agastache rugosa.BMC Plant Biol2026https://conductscience.org/library/papers/42420857PubMed
42,254,540Profiling heterologous 3' terminator-dependent expression variation in Aspergillus oryzae.Synth Syst Biotechnol2026https://conductscience.org/library/papers/42254540PubMed
42,185,385Directed mutagenesis of large multi-subunit protein complexes by plasmid sub-fragmentation.Sci Rep2026https://conductscience.org/library/papers/42185385PubMed

1 to 10 of 2,940

See all 2,940 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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