Paper · 33247285

Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH.

Messal HA and 9 others. Nat Protoc, 2021. Cited by 80 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH. Papers, record 33247285. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/33247285

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH.; PubMed
Journal: Nat Protoc; PubMed
Year: 2021; PubMed
Publication types: Journal Article, Research Support, Non-U.S. Gov't; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/33247285/; PubMed
DOI: https://doi.org/10.1038/s41596-020-00414-z; PubMed
The journal: https://conductscience.org/library/journal-index/101284307; PubMed
Citations, by iCite: 80; iCite
Relative citation ratio, by iCite: 4.13; iCite
Revised in PubMed: 2026-10-10; PubMed

Authors10

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename	ORCID iD
1	Messal HA	Hendrik A	https://orcid.org/0000-0003-2259-0286
2	Almagro J	Jorge	https://orcid.org/0000-0002-0652-6447
3	Zaw Thin M	May	https://orcid.org/0000-0003-0036-2776
4	Tedeschi A	Antonio
5	Ciccarelli A	Alessandro
6	Blackie L	Laura	https://orcid.org/0000-0001-7058-2382
7	Anderson KI	Kurt I
8	Miguel-Aliaga I	Irene
9	van Rheenen J	Jacco
10	Behrens A	Axel	https://orcid.org/0000-0002-1557-1143

Source PubMed

Topics19

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Antigens	Major topic
Fluorescent Antibody Technique	Major topic
Imaging, Three-Dimensional	Major topic
Microscopy, Confocal	Major topic
Animals
Drosophila
Epitopes
Female
Humans
Kidney
Lacrimal Apparatus
Liver
Lung
Male
Mammary Glands, Human
Mice
Organoids
Pancreas
Stomach

Source PubMed

Grants4

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes it	Agency	Country	Stated by
MC_UP_1102/3	Medical Research Council	United Kingdom	PubMed
FC001039	Medical Research Council	United Kingdom	PubMed
FC001039	Cancer Research UK	United Kingdom	PubMed
FC001039	Wellcome Trust	United Kingdom	PubMed

Source PubMed

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References61

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	32,235,925	Guidance for quantitative confocal microscopy.	Nat Protoc	2020	https://conductscience.org/library/papers/32235925	PubMed
2	30,134,179	Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.	Cell Rep	2018	https://conductscience.org/library/papers/30134179	PubMed
3	29,887,378	Next-Generation Machine Learning for Biological Networks.	Cell	2018	https://conductscience.org/library/papers/29887378	PubMed
4	30,397,326	Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.	Nat Methods	2018	https://conductscience.org/library/papers/30397326	PubMed
5	29,320,487	Multicolor quantitative confocal imaging cytometry.	Nat Methods	2018	https://conductscience.org/library/papers/29320487	PubMed
6	22,743,772	Fiji: an open-source platform for biological-image analysis.	Nat Methods	2012	https://conductscience.org/library/papers/22743772	PubMed
7	11,457,921	Antigen retrieval techniques: current perspectives.	J Histochem Cytochem	2001	https://conductscience.org/library/papers/11457921	PubMed
9	15,310,904	Optical sectioning deep inside live embryos by selective plane illumination microscopy.	Science	2004	https://conductscience.org/library/papers/15310904	PubMed
10	27,548,807	Shrinkage-mediated imaging of entire organs and organisms using uDISCO.	Nat Methods	2016	https://conductscience.org/library/papers/27548807	PubMed
11	24,412,654	Clearing pigmented insect cuticle to investigate small insects' organs in situ using confocal laser-scanning microscopy (CLSM).	Arthropod Struct Dev	2014	https://conductscience.org/library/papers/24412654	PubMed

1 to 10 of 61

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Cited by59

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 80 citations.

PMID	Title	Journal	Year	In Papers	Source
42,662,519	Current progress of labeling strategies in tissue clearing for large-scale biological visualization.	Front Cell Dev Biol	2026	https://conductscience.org/library/papers/42662519	PubMed
42,478,151	Quantitative computerized analysis demonstrates strongly compartmentalized tissue deformation patterns underlying mammalian heart tube formation.	Elife	2026	https://conductscience.org/library/papers/42478151	PubMed
42,247,300	Protocol for ultrafast immunolabeling and 3D imaging of whole organs and large tissues.	STAR Protoc	2026	https://conductscience.org/library/papers/42247300	PubMed
42,179,916	Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.	Comput Struct Biotechnol J	2026	https://conductscience.org/library/papers/42179916	PubMed
42,115,292	Transcriptional landscape of pulmonary artery endothelium reveals subpopulation- and disease-specific remodeling signatures.	Commun Biol	2026	https://conductscience.org/library/papers/42115292	PubMed
41,661,076	Myofibroblasts Induce Neuroplasticity to Promote Pancreatic Inflammation and Cancer Progression.	Cancer Discov	2026	https://conductscience.org/library/papers/41661076	PubMed
41,648,732	Improving gastrointestinal cancer therapy by uniting stakeholders.	ESMO Gastrointest Oncol	2024	https://conductscience.org/library/papers/41648732	PubMed
41,646,420	A modular tissue-clearing framework integrated with light-field microscopy enables rapid volumetric phenotyping of cardiac tissue.	Res Sq	2026	https://conductscience.org/library/papers/41646420	PubMed
41,472,314	Neural modulation of ocular immunity: Exploring a sympathetic-macrophage neuroimmune link in the human ciliary body.	J Anat	2026	https://conductscience.org/library/papers/41472314	PubMed
41,249,703	Bronchus-associated lymphoid tissue: a review of its development and function, including recent findings on the impact of environmental particulate exposure.	Clin Exp Med	2025	https://conductscience.org/library/papers/41249703	PubMed

1 to 10 of 59

See all 59 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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