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Paper · 33534838

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection.

Graham TGW and 9 others. PLoS One, 2021. Cited by 27 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection. Papers, record 33534838. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/33534838

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection.
PubMed
Journal
PLoS One
PubMed
Year
2021
PubMed
Publication types
Journal Article, Research Support, Non-U.S. Gov't
PubMed
Citations, by iCite
27
iCite
Relative citation ratio, by iCite
1.81
iCite
Revised in PubMed
2025-08-27
PubMed

Authors10

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Graham TGWThomas G Whttps://orcid.org/0000-0001-5189-4313
2Dugast-Darzacq CClairehttps://orcid.org/0000-0001-8602-3534
3Dailey GMGina M
4Wrynla XHXammy Hhttps://orcid.org/0000-0002-7532-8356
5Van Dis EErikhttps://orcid.org/0000-0003-4773-379X
6Esbin MNMeagan Nhttps://orcid.org/0000-0002-2157-3831
7Abidi AAbrar
8Stanley SASarah A
9Darzacq XXavier
10Tjian RRoberthttps://orcid.org/0000-0003-0539-8217

Source PubMed

Topics11

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
COVID-19Major topic
RNA, ViralMajor topic
Real-Time Polymerase Chain ReactionMajor topic
SARS-CoV-2Major topic
COVID-19 Nucleic Acid Testing
Chemical Precipitation
Coronavirus Nucleocapsid Proteins
Humans
Limit of Detection
Nasopharynx
Phosphoproteins

Source PubMed

Grants3

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated bySource
T32 GM008295NIGMS NIH HHSUnited StatesT32GM008295PubMed and NIHNIH RePORTER
T32 GM098218NIGMS NIH HHSUnited StatesT32GM098218PubMedNIH RePORTER
Howard Hughes Medical InstituteUnited StatesPubMedPubMed

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References25

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
132,492,531Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples.Int J Infect Dis2020https://conductscience.org/library/papers/32492531PubMed
332,790,779Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection.PLoS One2020https://conductscience.org/library/papers/32790779PubMed
41,579,465Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.Nucleic Acids Res1992https://conductscience.org/library/papers/1579465PubMed
58,074,881TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase.Biotechniques1994https://conductscience.org/library/papers/8074881PubMed
632,592,724Room-temperature-storable PCR mixes for SARS-CoV-2 detection.Clin Biochem2020https://conductscience.org/library/papers/32592724PubMed
732,555,529Blueprint for a pop-up SARS-CoV-2 testing lab.Nat Biotechnol2020https://conductscience.org/library/papers/32555529PubMed
932,570,810Rapid Direct Nucleic Acid Amplification Test without RNA Extraction for SARS-CoV-2 Using a Portable PCR Thermocycler.Genes (Basel)2020https://conductscience.org/library/papers/32570810PubMed
1032,883,809A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits.J Biol Chem2020https://conductscience.org/library/papers/32883809PubMed
1132,358,057Overcoming the bottleneck to widespread testing: a rapid review of nucleic acid testing approaches for COVID-19 detection.RNA2020https://conductscience.org/library/papers/32358057PubMed
1232,706,827Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA.PLoS One2020https://conductscience.org/library/papers/32706827PubMed

1 to 10 of 25

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Cited by19

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 27 citations.

PMIDTitleJournalYearIn PapersSource
40,763,288Open educational resources for distributed hands-on teaching in molecular biology.PLoS One2025https://conductscience.org/library/papers/40763288PubMed
40,465,030Detection and molecular characterisation of subtype B avian metapneumovirus in commercial chickens and co-infection with bacteria pathogens in Nigeria.Trop Anim Health Prod2025https://conductscience.org/library/papers/40465030PubMed
40,244,855Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples.STAR Protoc2025https://conductscience.org/library/papers/40244855PubMed
38,322,940Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection.Heliyon2024https://conductscience.org/library/papers/38322940PubMed
38,271,448An Open One-Step RT-qPCR for SARS-CoV-2 detection.PLoS One2024https://conductscience.org/library/papers/38271448PubMed
38,212,439A high-performance polymer composite column for coronavirus nucleic acid purification.Sci Rep2024https://conductscience.org/library/papers/38212439PubMed
37,351,183The quality of commercial SARS-CoV-2 nucleic acid tests in Ecuador: lessons from COVID-19 pandemic for advancing social equity through microbiology.Front Cell Infect Microbiol2023https://conductscience.org/library/papers/37351183PubMed
37,346,701Graph data science and machine learning for the detection of COVID-19 infection from symptoms.PeerJ Comput Sci2023https://conductscience.org/library/papers/37346701PubMed
35,746,782Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests.Viruses2022https://conductscience.org/library/papers/35746782PubMed
35,642,647CRISPR-based point-of-care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS-CoV-2 detection.J Biochem Mol Toxicol2022https://conductscience.org/library/papers/35642647PubMed

1 to 10 of 19

See all 19 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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