Paper · 344137

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Bolivar F and 7 others. Gene, 1977. Cited by 5,067 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

Cite this record

ConductScience Library. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Papers, record 344137. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/344137

On this page

Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.; PubMed
Journal: Gene; PubMed
Year: 1977; PubMed
Publication types: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/344137/; PubMed
The journal: https://conductscience.org/library/journal-index/7706761; PubMed
Citations, by iCite: 5,067; iCite
Relative citation ratio, by iCite: 126; iCite
Revised in PubMed: 2024-01-04; PubMed

Authors8

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename
1	Bolivar F	F
2	Rodriguez RL	R L
3	Greene PJ	P J
4	Betlach MC	M C
5	Heyneker HL	H L
6	Boyer HW	H W
7	Crosa JH	J H
8	Falkow S	S

Source PubMed

Topics9

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Plasmids	Major topic
Recombination, Genetic	Major topic
Ampicillin
Conjugation, Genetic
DNA, Bacterial
DNA, Recombinant
Escherichia coli
Tetracycline
Transformation, Bacterial

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References0

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

None in this release.

Cited by2,536

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 5,067 citations.

PMID	Title	Journal	Year	In Papers	Source
42,287,315	Immunopharmacological and structural design of a polyprotein based multi epitope vaccine against Powassan virus.	Naunyn Schmiedebergs Arch Pharmacol	2026	https://conductscience.org/library/papers/42287315	PubMed
42,092,359	Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods.	Cell Rep Methods	2026	https://conductscience.org/library/papers/42092359	PubMed
42,079,886	Opinion of the Scientific Panel on genetically modified organisms [GMO] and the opinion of the Scientific Panel on additives and products or substances used in animal feed [FEEDAP] on the safety and efficacy of the enzyme preparation Phytase SP 1002 (3-phytase) for use as feed additive for piglets, pigs for fattening, sows, chickens for fattening, turkeys and laying hens.	EFSA J	2006	https://conductscience.org/library/papers/42079886	PubMed
41,182,907	A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity.	Microbiology (Reading)	2025	https://conductscience.org/library/papers/41182907	PubMed
41,179,728	Evolution of hepatology practice in Mexico and Latin America: From biochemical markers to genomic medicine.	World J Hepatol	2025	https://conductscience.org/library/papers/41179728	PubMed
41,168,947	The bacterial MRE11-RAD50 and DNA2-WRN homologs process replication forks at distinct and separate loci on the chromosome.	FEBS Lett	2026	https://conductscience.org/library/papers/41168947	PubMed
40,920,826	Topo IV is required to allow replisomes to converge and complete replication on the chromosome.	PLoS Genet	2025	https://conductscience.org/library/papers/40920826	PubMed
40,866,349	Vibrio cholerae motility is associated with inter-animal transmission.	Nat Commun	2025	https://conductscience.org/library/papers/40866349	PubMed
40,622,926	pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2.	PLoS One	2025	https://conductscience.org/library/papers/40622926	PubMed
40,603,587	Antagonistic effect of kanamycin on kanamycin-resistant Escherichia coli infection by BtuB-targeting bacteriophages.	Arch Virol	2025	https://conductscience.org/library/papers/40603587	PubMed

1 to 10 of 2,536

See all 2,536 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

For software

Read this paper from software.

The record and each of its lists are JSON, twenty rows a page. No key.

GET https://conductscience.org/api/papers/v1/344137

GET https://conductscience.org/api/papers/v1/344137/cited_by?page=2

API reference For AI assistants