Paper · 36912542

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes.

Lewis KJ and 5 others. J Vis Exp, 2023. Cited by 7 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes. Papers, record 36912542. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/36912542

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes.; PubMed
Journal: J Vis Exp; PubMed
Year: 2023; PubMed
Publication types: Journal Article, Video-Audio Media; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/36912542/; PubMed
DOI: https://doi.org/10.3791/64366; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC13536361/; PubMed
The journal: https://conductscience.org/library/journal-index/101313252; PubMed
Citations, by iCite: 7; iCite
Relative citation ratio, by iCite: 1.35; iCite
Revised in PubMed: 2026-09-03; PubMed

Authors6

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename
1	Lewis KJ	Karl J
2	Boorman-Padgett JF	James F
3	Castaneda M	Macy
4	Spray DC	David C
5	Thi MM	Mia M
6	Schaffler MB	Mitchell B

Source PubMed

Topics9

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Osteocytes	Major topic
Mechanotransduction, Cellular	Major topic
Animals
Mice
Calcium
Calcium Signaling
Coloring Agents
Intravital Microscopy
Stress, Mechanical

Source PubMed

Grants3

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes it	Agency	Country	NIH project	Stated by	Source
R01 AR041210	NIAMS NIH HHS	United States	R01AR041210	PubMed	NIH RePORTER
R01 AR057139	NIAMS NIH HHS	United States	R01AR057139	PubMed	NIH RePORTER
R01 AR070547	NIAMS NIH HHS	United States	R01AR070547	PubMed	NIH RePORTER

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References36

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	31,009,584	Tissue Clearing and Its Application to Bone and Dental Tissues.	J Dent Res	2019	https://conductscience.org/library/papers/31009584	PubMed
2	20,617,941	Osteocyte mechanobiology and pericellular mechanics.	Annu Rev Biomed Eng	2010	https://conductscience.org/library/papers/20617941	PubMed
3	16,175,387	Isolated primary osteocytes express functional gap junctions in vitro.	Cell Tissue Res	2006	https://conductscience.org/library/papers/16175387	PubMed
4	19,539,797	Identification of differentially expressed genes between osteoblasts and osteocytes.	Bone	2009	https://conductscience.org/library/papers/19539797	PubMed
5	19,898,485	Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators.	Nat Methods	2009	https://conductscience.org/library/papers/19898485	PubMed
6	17,301,958	Oscillating fluid flow activation of gap junction hemichannels induces ATP release from MLO-Y4 osteocytes.	J Cell Physiol	2007	https://conductscience.org/library/papers/17301958	PubMed
7	23,868,258	Ultrasensitive fluorescent proteins for imaging neuronal activity.	Nature	2013	https://conductscience.org/library/papers/23868258	PubMed
8	29,581,909	Mechanically induced Ca2+ oscillations in osteocytes release extracellular vesicles and enhance bone formation.	Bone Res	2018	https://conductscience.org/library/papers/29581909	PubMed
9	22,750,013	Calcium response in osteocytic networks under steady and oscillatory fluid flow.	Bone	2012	https://conductscience.org/library/papers/22750013	PubMed
10	24,347,610	In situ intracellular calcium oscillations in osteocytes in intact mouse long bones under dynamic mechanical loading.	FASEB J	2014	https://conductscience.org/library/papers/24347610	PubMed

1 to 10 of 36

See all 36 references

Cited by6

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 7 citations.

PMID	Title	Journal	Year	In Papers	Source
42,812,220	Intravital Depletion of Cholesterol Reduces Membrane Dynamics and Increases Mechanosensitivity in Osteocytes In Vivo.	bioRxiv	2026	https://conductscience.org/library/papers/42812220	PubMed
42,538,975	Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone.	bioRxiv	2026	https://conductscience.org/library/papers/42538975	PubMed
41,301,453	Integrating 3D Osteocyte Culture, Microgravity Simulation, and Fluid Flow Reveals Mechanisms of Osteocyte Mechanosensation and Calcium Signaling Altered by Disuse.	Biomolecules	2025	https://conductscience.org/library/papers/41301453	PubMed
40,845,105	Cholinergic regulation of osteocyte mechanobiology: A paradigm for bone adaptation.	Sci Adv	2025	https://conductscience.org/library/papers/40845105	PubMed
40,410,950	Combined Effects of Mechanical Loading and Piezo1 Chemical Activation on 22-Months-Old Female Mouse Bone Adaptation.	Aging Cell	2025	https://conductscience.org/library/papers/40410950	PubMed
40,061,293	Electro-spun piezoelectric PLLA smart composites as a scaffold on bone fracture: A review.	Regen Ther	2025	https://conductscience.org/library/papers/40061293	PubMed

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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