Paper · 414220

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Alwine JC and 2 others. Proc Natl Acad Sci U S A, 1977. Cited by 1,925 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Papers, record 414220. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/414220

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.; PubMed
Journal: Proc Natl Acad Sci U S A; PubMed
Year: 1977; PubMed
Publication types: Journal Article, Research Support, U.S. Gov't, P.H.S.; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/414220/; PubMed
DOI: https://doi.org/10.1073/pnas.74.12.5350; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC431715/; PubMed
The journal: https://conductscience.org/library/journal-index/7505876; PubMed
Citations, by iCite: 1,925; iCite
Relative citation ratio, by iCite: 40.1; iCite
Revised in PubMed: 2019-12-10; PubMed

Authors3

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename
1	Alwine JC	J C
2	Kemp DJ	D J
3	Stark GR	G R

Source PubMed

Topics12

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
RNA	Major topic
Animals
Autoradiography
Cellulose
Chemical Phenomena
Chemistry
Diazonium Compounds
Drosophila melanogaster
Electrophoresis, Agar Gel
Nucleic Acid Hybridization
RNA, Ribosomal
Solubility

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References19

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
2	13,903,029	[Production of protein-cellulose complexes (immunosorbents) in suspension forms with the capacity for absorption of large amounts of antibodies].	Biokhimiia	1961	https://conductscience.org/library/papers/13903029	PubMed
3	13,628,816	[Isolation of pure antibodies with the aid of fixed on cellulose antigens and studies on their properties].	Biokhimiia	1959	https://conductscience.org/library/papers/13628816	PubMed
4	881,736	Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.	J Mol Biol	1977	https://conductscience.org/library/papers/881736	PubMed
5	61,066	Amplification and characterization of a beta-globin gene synthesized in vitro.	Cell	1976	https://conductscience.org/library/papers/61066	PubMed
6	412,196	Translation of Drosophila melanogaster sequences in Escherichia coli.	Proc Natl Acad Sci U S A	1977	https://conductscience.org/library/papers/412196	PubMed
7	402,221	R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.	Cell	1977	https://conductscience.org/library/papers/402221	PubMed
8	269,380	Spliced segments at the 5' terminus of adenovirus 2 late mRNA.	Proc Natl Acad Sci U S A	1977	https://conductscience.org/library/papers/269380	PubMed
9	4,216,403	A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.	Cell	1974	https://conductscience.org/library/papers/4216403	PubMed
10	4,594,039	Construction of biologically functional bacterial plasmids in vitro.	Proc Natl Acad Sci U S A	1973	https://conductscience.org/library/papers/4594039	PubMed
11	167,982	Nucleic acid hybridization using DNA covalently coupled to cellulose.	Cell	1975	https://conductscience.org/library/papers/167982	PubMed

1 to 10 of 19

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Cited by709

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 1,925 citations.

PMID	Title	Journal	Year	In Papers	Source
42,911,496	Detection and Quantification of RNA Using Near-Infrared Northern Blot.	Methods Mol Biol	2027	https://conductscience.org/library/papers/42911496	PubMed
42,367,211	RNA Detection Technologies: A MethodCentric Guide to Principles and Reproducibility.	Bio Protoc	2026	https://conductscience.org/library/papers/42367211	PubMed
42,098,734	10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models.	Genome Biol	2026	https://conductscience.org/library/papers/42098734	PubMed
42,079,126	Ribosome biogenesis bottlenecks reveal vulnerabilities in cancer.	bioRxiv	2026	https://conductscience.org/library/papers/42079126	PubMed
42,071,138	Microarrays and the Dawn of Omics Data Analysis.	Adv Exp Med Biol	2026	https://conductscience.org/library/papers/42071138	PubMed
42,055,626	Cancer Cytogenetics: Deep Roots, New Branches in the Age of Omics.	Cancer Genomics Proteomics	2026	https://conductscience.org/library/papers/42055626	PubMed
41,998,316	Tools and tactics for studying alternative splicing.	Nat Rev Genet	2026	https://conductscience.org/library/papers/41998316	PubMed
41,979,651	[Half a century of gene expression analysis : The path to modern spatial transcriptomics in autopsy tissue].	Pathologie (Heidelb)	2026	https://conductscience.org/library/papers/41979651	PubMed
41,626,325	Transcriptomics in solanaceous crop improvement: advances and opportunities.	Front Plant Sci	2025	https://conductscience.org/library/papers/41626325	PubMed
41,478,915	Fluorescent Probes for the Quantification of RNA by Real-Time qPCR.	Methods Mol Biol	2026	https://conductscience.org/library/papers/41478915	PubMed

1 to 10 of 709

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Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

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ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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