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Paper · 61588

A general method for cloning eukaryotic structural gene sequences.

Higuchi R and 3 others. Proc Natl Acad Sci U S A, 1976. Cited by 98 papers, by iCite.

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ConductScience Library. A general method for cloning eukaryotic structural gene sequences. Papers, record 61588. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/61588

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
A general method for cloning eukaryotic structural gene sequences.
PubMed
Journal
Proc Natl Acad Sci U S A
PubMed
Year
1976
PubMed
Publication types
Journal Article, Research Support, U.S. Gov't, P.H.S.
PubMed
Citations, by iCite
98
iCite
Relative citation ratio, by iCite
2.19
iCite
Revised in PubMed
2019-05-01
PubMed

Authors4

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Higuchi RR
2Paddock GVG V
3Wall RR
4Salser WW

Source PubMed

Topics13

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
DNAMajor topic
Extrachromosomal InheritanceMajor topic
GenesMajor topic
Genetic EngineeringMajor topic
PlasmidsMajor topic
RNA, MessengerMajor topic
Base Sequence
DNA, Bacterial
Escherichia coli
Globins
Nucleic Acid Hybridization
RNA-Directed DNA Polymerase
Transformation, Genetic

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References24

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
11,101,952A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.Biochemistry1975https://conductscience.org/library/papers/1101952PubMed
21,105,573Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proc Natl Acad Sci U S A1975https://conductscience.org/library/papers/1105573PubMed
31,107,072Investigation of the organization of mammalian chromosomes at the DNA sequence level.Fed Proc1976https://conductscience.org/library/papers/1107072PubMed
411,643,293Recombinant DNA: NIH sets strict rules to launch new technology.Science1975https://conductscience.org/library/papers/11643293PubMed
514,114,848ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.J Biol Chem1964https://conductscience.org/library/papers/14114848PubMed
6165,498Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.Proc Natl Acad Sci U S A1975https://conductscience.org/library/papers/165498PubMed
74,139,714Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.Proc Natl Acad Sci U S A1974https://conductscience.org/library/papers/4139714PubMed
84,594,039Construction of biologically functional bacterial plasmids in vitro.Proc Natl Acad Sci U S A1973https://conductscience.org/library/papers/4594039PubMed
94,600,264Replication and transcription of eukaryotic DNA in Escherichia coli.Proc Natl Acad Sci U S A1974https://conductscience.org/library/papers/4600264PubMed
104,604,359DNA sequencing techniques.Annu Rev Biochem1974https://conductscience.org/library/papers/4604359PubMed

1 to 10 of 24

See all 24 references

Cited by33

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 98 citations.

PMIDTitleJournalYearIn PapersSource
22,450,538Functional cDNA expression cloning: pushing it to the limit.Proc Jpn Acad Ser B Phys Biol Sci2012https://conductscience.org/library/papers/22450538PubMed
15,668,518A new 5' terminal murine GAPDH exon identified using 5'RACE LaNe.Mol Biotechnol2005https://conductscience.org/library/papers/15668518PubMed
7,017,712Nucleotide sequence of cloned rat serum albumin messenger RNA.Proc Natl Acad Sci U S A1981https://conductscience.org/library/papers/7017712PubMed
6,784,124Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.Proc Natl Acad Sci U S A1980https://conductscience.org/library/papers/6784124PubMed
6,294,624Neuraminidase gene from the early Asian strain of human influenza virus, A/RI/5-/57 (H2N2).Nucleic Acids Res1982https://conductscience.org/library/papers/6294624PubMed
6,167,988Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin.Proc Natl Acad Sci U S A1981https://conductscience.org/library/papers/6167988PubMed
6,166,9215'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.Nucleic Acids Res1981https://conductscience.org/library/papers/6166921PubMed
6,152,853Chinese hamster polyadenylated messenger ribonucleic acid: relationship to non-polyadenylated sequences and relative conservation during messenger ribonucleic acid processing.Mol Cell Biol1981https://conductscience.org/library/papers/6152853PubMed
3,018,493Quantitative assessment of actin transcript number in eggs, embryos, and tube feet of the sea star Pisaster ochraceus.Mol Cell Biol1985https://conductscience.org/library/papers/3018493PubMed
2,826,120Molecular analysis of heritable mouse mutations.Environ Health Perspect1987https://conductscience.org/library/papers/2826120PubMed

1 to 10 of 33

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Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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