Paper · 68686

A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Sedmak JJ and 1 other. Anal Biochem, 1977. Cited by 2,253 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Papers, record 68686. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/68686

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.; PubMed
Journal: Anal Biochem; PubMed
Year: 1977; PubMed
Publication types: Journal Article, Research Support, U.S. Gov't, P.H.S.; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/68686/; PubMed
DOI: https://doi.org/10.1016/0003-2697(77)90428-6; PubMed
The journal: https://conductscience.org/library/journal-index/0370535; PubMed
Citations, by iCite: 2,253; iCite
Relative citation ratio, by iCite: 66.1; iCite
Revised in PubMed: 2024-01-09; PubMed

Authors2

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename	Kind	ORCID iD
1	Sedmak JJ	J J
2	Grossberg SE	S E

Source PubMed

Topics8

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Proteins	Major topic
Rosaniline Dyes	Major topic
Buffers
Methods
Molecular Weight
Peptides
Serum Albumin, Bovine
Staining and Labeling

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References0

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

None in this release.

Cited by581

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 2,253 citations.

PMID	Title	Journal	Year	In Papers	Source
42,702,726	5-Aminolevulinic acid promotes cell division and young fruit development by suppressing the PpARF9-PpGH3.1 auxin-conjugation module in peach.	Mol Hortic	2026	https://conductscience.org/library/papers/42702726	PubMed
42,368,503	Comprehensive Chemical Profiling and Assessment of In-Vitro Antioxidant and Antidiabetic Activities of Polysaccharides Derived from Allium cepa and Allium sativum.	J Pharmacopuncture	2026	https://conductscience.org/library/papers/42368503	PubMed
41,978,095	Structural Characterization and Anti-Colitis Mechanisms of Polygonatum sibiricum Polysaccharides via Modulation of Neutrophil Extracellular Traps (NETs)-Macrophage Crosstalk.	Nutrients	2026	https://conductscience.org/library/papers/41978095	PubMed
41,953,295	Landfill leachate treatment by the symbiotic system of bacteria and microalgae: high-efficiency and low-consumption green biological treatment system.	RSC Adv	2026	https://conductscience.org/library/papers/41953295	PubMed
41,720,903	Kaolin particle film reduces drought stress in grapevines and regulates photosynthesis and antioxidant capacity.	Sci Rep	2026	https://conductscience.org/library/papers/41720903	PubMed
41,624,581	Polysaccharides from Pleurotus eryngii: sequential extraction and structural characterization.	Food Chem X	2026	https://conductscience.org/library/papers/41624581	PubMed
41,493,009	Autoclaved Peanuts Exhibit Reduced Immunoglobulin E Binding and Improved Oral Tolerability.	Allergy	2026	https://conductscience.org/library/papers/41493009	PubMed
41,122,407	Valorization of Channa striatus waste skin for developing marine collagen peptide based hydrogels for potential wound dressings.	RSC Adv	2025	https://conductscience.org/library/papers/41122407	PubMed
41,064,394	Evaluation of the Concentration of Ethanol as a Vehicle for the Administration of Resveratrol Measured Through Its Antioxidant Effect in the Hippocampus of Wistar Rats.	J Nutr Metab	2025	https://conductscience.org/library/papers/41064394	PubMed
41,062,680	The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection.	Sci Rep	2025	https://conductscience.org/library/papers/41062680	PubMed

1 to 10 of 581

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Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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