Paper · 73185

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

McMaster GK and 1 other. Proc Natl Acad Sci U S A, 1977. Cited by 2,457 papers, by iCite.

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ConductScience Library. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Papers, record 73185. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/73185

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.; PubMed
Journal: Proc Natl Acad Sci U S A; PubMed
Year: 1977; PubMed
Publication types: Journal Article; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/73185/; PubMed
DOI: https://doi.org/10.1073/pnas.74.11.4835; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC432050/; PubMed
The journal: https://conductscience.org/library/journal-index/7505876; PubMed
Citations, by iCite: 2,457; iCite
Relative citation ratio, by iCite: 53.5; iCite
Revised in PubMed: 2019-05-01; PubMed

Authors2

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename	Kind	ORCID iD
1	McMaster GK	G K
2	Carmichael GG	G G

Source PubMed

Topics10

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
DNA, Single-Stranded	Major topic
RNA	Major topic
Acridines
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Glyoxal
Molecular Weight
Nucleic Acid Denaturation
Photography
Staining and Labeling

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References37

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	1,052,528	A new method of DNA denaturation mapping.	Nucleic Acids Res	1975	https://conductscience.org/library/papers/1052528	PubMed
2	1,066,674	Electron microscopy of DNA crosslinked with trimethylpsoralen: test of the secondary structure of eukaryotic inverted repeat sequences.	Proc Natl Acad Sci U S A	1976	https://conductscience.org/library/papers/1066674	PubMed
3	10,793,712	Internal structure of the chromatin subunit.	Nucleic Acids Res	1974	https://conductscience.org/library/papers/10793712	PubMed
4	1,138,234	A new method for mapping nucleic acid sequence homology by electron microscopy.	Nucleic Acids Res	1975	https://conductscience.org/library/papers/1138234	PubMed
5	1,253,794	Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels.	Eur J Biochem	1976	https://conductscience.org/library/papers/1253794	PubMed
6	1,259,158	Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.	Anal Biochem	1976	https://conductscience.org/library/papers/1259158	PubMed
7	13,761,054	Structural considerations in the interaction of DNA and acridines.	J Mol Biol	1961	https://conductscience.org/library/papers/13761054	PubMed
8	13,768,614	Studies on the acridine orange staining of two purified RNA viruses: poliovirus and tobacco mosaic virus.	Virology	1961	https://conductscience.org/library/papers/13768614	PubMed
9	13,929,834	The structure of the DNA-acridine complex.	Proc Natl Acad Sci U S A	1963	https://conductscience.org/library/papers/13929834	PubMed
10	16,590,488	AGGREGATION OF DYES BOUND TO POLYANIONS.	Proc Natl Acad Sci U S A	1959	https://conductscience.org/library/papers/16590488	PubMed

1 to 10 of 37

See all 37 references

Cited by941

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 2,457 citations.

PMID	Title	Journal	Year	In Papers	Source
42,923,621	The Dual-Ribozyme RNA Ladder and RNA-Calibrated ssDNA Marker System for Inexpensive RNA Size Standards.	ACS Omega	2026	https://conductscience.org/library/papers/42923621	PubMed
41,551,587	Protrichocysts: a hybrid defense extrusive organelle bridging mechanical projection and chemical secretion in ciliates.	Curr Res Microb Sci	2026	https://conductscience.org/library/papers/41551587	PubMed
41,353,232	A rapid method of evaluating cytotoxic drug efficacy using sub-cellular fluctuation imaging.	Sci Rep	2025	https://conductscience.org/library/papers/41353232	PubMed
40,418,501	Advanced fixation techniques for high-sensitivity molecular imaging: effectiveness of glyoxal fixation for immunostaining.	Anat Sci Int	2025	https://conductscience.org/library/papers/40418501	PubMed
38,554,110	Live-cell imaging of human apurinic/apyrimidinic endonuclease 1 in the nucleus and nucleolus using a chaperone@DNA probe.	Nucleic Acids Res	2024	https://conductscience.org/library/papers/38554110	PubMed
38,285,384	Detection of dsRNA by Acridine Orange Staining.	Methods Mol Biol	2024	https://conductscience.org/library/papers/38285384	PubMed
37,894,920	Chemical Insights into Oxidative and Nitrative Modifications of DNA.	Int J Mol Sci	2023	https://conductscience.org/library/papers/37894920	PubMed
37,450,597	Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research.	Sci Adv	2023	https://conductscience.org/library/papers/37450597	PubMed
37,235,378	Effect of Monocerin, a Fungal Secondary Metabolite, on Endothelial Cells.	Toxins (Basel)	2023	https://conductscience.org/library/papers/37235378	PubMed
35,980,233	High-throughput force measurement of individual kinesin-1 motors during multi-motor transport.	Nanoscale	2022	https://conductscience.org/library/papers/35980233	PubMed

1 to 10 of 941

See all 941 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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