Age-Related Differences in Exercise-Induced Immune Responses in Soccer Players
NCT07933770, Not yet recruiting, Poznan University of Physical Education
Trial 9
As registered on ClinicalTrials.gov, in the graph's capture of the registry.
| Field | Value | Source |
|---|---|---|
| Title | Age-Related Differences in Exercise-Induced Immune Responses in Soccer Players | ClinicalTrials.gov |
| NCT number | NCT07933770 | ClinicalTrials.gov |
| Status | Not yet recruiting | ClinicalTrials.gov |
| Enrollment | 45 participants, estimated | ClinicalTrials.gov |
| Lead sponsor | Poznan University of Physical Education | ClinicalTrials.gov |
| Conditions | Exercise Training | ClinicalTrials.gov |
| Interventions | Beep test | ClinicalTrials.gov |
| First posted | 2026-11-12 | ClinicalTrials.gov |
| Last updated | 2026-11-12 | ClinicalTrials.gov |
Registered outcomes 18
Each outcome the trial registered, primary first, in ClinicalTrials.gov's words.
| List | Outcome | Time frame | Description | Source |
|---|---|---|---|---|
| Primary | Exercise-Induced Change in Circulating Lipopolysaccharide (LPS) Concentration | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Circulating lipopolysaccharide (LPS) concentration will be measured using an ELISA assay in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in circulating LPS concentrations and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in Circulating Lipopolysaccharide binding protein (LBP) Concentration | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Circulating lipopolysaccharide binding protein (LBP) concentration will be measured using an ELISA assay in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in circulating LPS concentrations and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in Circulating Soluble CD14 (sCD14) Concentration | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Circulating Soluble CD14 (sCD14) concentration will be measured using an ELISA assay in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in circulating LPS concentrations and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in Circulating Soluble Toll-Like Receptor 4 (sTLR4) Concentration | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Circulating Soluble Toll-Like Receptor 4 (sTLR4) concentration will be measured using an ELISA assay in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in circulating LPS concentrations and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in Circulating Soluble Toll-Like Receptor 2 (sTLR2) Concentration | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Circulating Soluble Toll-Like Receptor 2 (sTLR2) concentration will be measured using an ELISA assay in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in circulating LPS concentrations and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in CD14+ Monocyte Population | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | The proportion of CD14+ monocytes will be assessed by flow cytometry in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in the CD14+ monocyte population and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in CD16+ Monocyte Population | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | The proportion of CD16+ monocytes will be assessed by flow cytometry in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in the CD14+ monocyte population and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in TLR2 Monocyte Population | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | The proportion of TLR2 monocytes will be assessed by flow cytometry in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in the CD14+ monocyte population and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in TLR4 Monocyte Population | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | The proportion of TLR2 monocytes will be assessed by flow cytometry in blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in the CD14+ monocyte population and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in TLR2 Gene Expression in Peripheral Blood Mononuclear Cells | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | TLR2 gene expression will be assessed in peripheral blood mononuclear cells (PBMCs) isolated from blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). TLR2 gene expression will be quantified using real-time quantitative PCR (RT-qPCR) and normalized to the reference gene GUSB. The outcome will be used to assess exercise-induced changes in TLR2 gene expression and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Change in TLR2 Gene Expression in Peripheral Blood Mononuclear Cells | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | TLR4 gene expression will be assessed in peripheral blood mononuclear cells (PBMCs) isolated from blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). TLR2 gene expression will be quantified using real-time quantitative PCR (RT-qPCR) and normalized to the reference gene GUSB. The outcome will be used to assess exercise-induced changes in TLR4 gene expression and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Primary | Exercise-Induced Changes in Complete Blood Count Parameters | Baseline (before exercise, immediately after exercise, and 1 hour after exercise). | Complete blood count parameters, including hemoglobin, hematocrit, erythrocyte count, leukocyte count, granulocyte count, lymphocyte count, monocyte count, platelet count, MCV, MCH, MCHC, and RDW, will be assessed in venous blood samples collected before exercise (PRE), immediately after exercise (POST), and 1 hour after exercise (REC). Measurements will be performed during a single study session involving a maximal 20-meter shuttle run test (Beep Test). The outcome will be used to assess exercise-induced changes in hematological parameters and to determine whether these responses differ among three age groups of soccer players: 14-15 years, 16-17 years, and 18 years and older. | ClinicalTrials.gov |
| Secondary | Body Mass | Baseline - in the morning | Body mass will be measured before the exercise test. Body mass will be reported in kilograms (kg). | ClinicalTrials.gov |
| Secondary | Dietary Intake Assessed Using a Food Diary | The day before exercise test. | Dietary intake will be assessed using a food diary completed on the day preceding each exercise test. Total energy intake and macronutrient intake, including protein, carbohydrate, and fat, will be calculated from the dietary records. | ClinicalTrials.gov |
| Secondary | Body Fat Percentage | Baseline - in the morning | Body fat percentage will be measured before the exercise test. Body fat will be reported in percentage (%). | ClinicalTrials.gov |
| Secondary | Fat-Free Mass | Baseline - in the morning | Fat-Free Mass will be measured before the exercise test. Fat-Free Mass be reported in percentage (%). | ClinicalTrials.gov |
| Secondary | Total body water | Baseline - in the morning | Total body water will be assessed before the exercise test at baseline. Total body water will be reported in percentage (%). | ClinicalTrials.gov |
| Secondary | Height | Baseline - in the morning | Height will be assessed before the exercise test at baseline. Height will be reported in centimeters \[cm\]. | ClinicalTrials.gov |
Outcomes 0
Each outcome the trial posted results for, in ClinicalTrials.gov's words.
| Type | Outcome | Time frame | Population | Units | Measured as | Source |
|---|
Results by group 0
Each measurement the trial posted, group by group, in the words and numbers it posted.
| Outcome | Group | Category | Value | Spread | Units | Source |
|---|
Participant flow 0
How many started, completed or left each group, period by period.
| Period | Milestone | Group | Participants | Units counted | Source |
|---|
Adverse events 0
Each adverse event the trial posted, group by group: the participants affected out of those at risk.
| Type | Event | Organ system | Group | Affected | At risk | Events | Source |
|---|
Adverse event totals 0
The totals the trial posted for each group.
| Total | Group | Affected | At risk | Source |
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Papers 0
The papers the trial lists on ClinicalTrials.gov.
| PMID | Listed as | Source |
|---|
Grants 0
The grants NIH RePORTER links to the trial, newest funded first: NIH's, and some of AHRQ's, CDC's, FDA's and VA's. Each shows its title, principal investigators, organization and funder in its newest year.
| Project | Title | Principal investigators | Organization | Funder | Fiscal years | Source |
|---|
Trial comparisons 0
Comparisons of the trial's two groups, calculated by ConductScience from the counts it posted.
| Comparison | Source |
|---|
Tests 0
The ConductCognition tests this trial's registered outcomes name, as ScienceGraph matched them in trial-tests-2026-11-12-0.2.1.
| Test | Named as | Source |
|---|
Source 5
| Field | Value | Source |
|---|---|---|
| Release | trials-2026-11-12-0.4.0 | ClinicalTrials.gov |
| Release manifest SHA-256 | 0f4a8d8c48aee2a7573ae0fa94ecbeab70c1adbe449bbc95c424bc7d3e97b9d4 | ClinicalTrials.gov |
| Registry capture | 2026-11-12 | ClinicalTrials.gov |
| Results copy, AACT | 2026-03-23 | ClinicalTrials.gov |
| NIH clinical studies file | 2026-11-13 | ClinicalTrials.gov |
Release trials-2026-11-12-0.4.0
ClinicalTrials.gov's posted results, through CTTI's AACT: ClinicalTrials.gov data via Aggregate Analysis of ClinicalTrials.gov (AACT), CTTI. CTTI makes its materials freely available and requests acknowledgement. https://aact.ctti-clinicaltrials.org/documentation/299 ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.
ClinicalTrials.gov: none stated ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.
NIH RePORTER's clinical studies and project files: Public domain (NIH RePORTER)