Paper · 22123741

A versatile element for gene addition in bacterial chromosomes.

Sibley MH and 1 other. Nucleic Acids Res, 2012. Cited by 19 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. A versatile element for gene addition in bacterial chromosomes. Papers, record 22123741. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/22123741

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: A versatile element for gene addition in bacterial chromosomes.; PubMed
Journal: Nucleic Acids Res; PubMed
Year: 2012; PubMed
Publication types: Journal Article, Validation Study; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/22123741/; PubMed
DOI: https://doi.org/10.1093/nar/gkr1085; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC3273789/; PubMed
The journal: https://conductscience.org/library/journal-index/0411011; PubMed
Citations, by iCite: 19; iCite
Relative citation ratio, by iCite: 0.47; iCite
Revised in PubMed: 2021-10-21; PubMed

Authors2

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename	Kind	ORCID iD
1	Sibley MH	Marion H
2	Raleigh EA	Elisabeth A

Source PubMed

Topics12

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Chromosomes, Bacterial	Major topic
DNA Transposable Elements	Major topic
Gene Targeting	Major topic
Cloning, Molecular
DNA Primers
Escherichia coli
Genetic Vectors
Promoter Regions, Genetic
Protein Biosynthesis
Rhamnose
Transcription, Genetic
beta-Galactosidase

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References33

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	10,610,816	Genetic system for reversible integration of DNA constructs and lacZ gene fusions into the Escherichia coli chromosome.	Plasmid	2000	https://conductscience.org/library/papers/10610816	PubMed
2	12,771,385	Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate.	Proc Natl Acad Sci U S A	2003	https://conductscience.org/library/papers/12771385	PubMed
3	3,074,013	Cloning type-II restriction and modification genes.	Gene	1988	https://conductscience.org/library/papers/3074013	PubMed
4	1,718,749	Analysis of the complex transcription termination region of the Escherichia coli rrnB gene.	Eur J Biochem	1991	https://conductscience.org/library/papers/1718749	PubMed
5	9,628,366	Dependence of McrBC cleavage on distance between recognition elements.	Biol Chem	1998	https://conductscience.org/library/papers/9628366	PubMed
6	11,591,683	Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria.	J Bacteriol	2001	https://conductscience.org/library/papers/11591683	PubMed
7	21,256,136	Probing cellular processes with oligo-mediated recombination and using the knowledge gained to optimize recombineering.	J Mol Biol	2011	https://conductscience.org/library/papers/21256136	PubMed
8	19,633,652	Programming cells by multiplex genome engineering and accelerated evolution.	Nature	2009	https://conductscience.org/library/papers/19633652	PubMed
9	17,341,463	USER friendly DNA engineering and cloning method by uracil excision.	Nucleic Acids Res	2007	https://conductscience.org/library/papers/17341463	PubMed
10	16,646,962	Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event.	BMC Microbiol	2006	https://conductscience.org/library/papers/16646962	PubMed

1 to 10 of 33

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Cited by12

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 19 citations.

PMID	Title	Journal	Year	In Papers	Source
41,870,091	Tunable low-rate genomic recombination with Cre-lox in Escherichia coli: a versatile tool for anoxic environmental biosensing and synthetic biology.	Appl Environ Microbiol	2026	https://conductscience.org/library/papers/41870091	PubMed
39,119,921	Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor.	Nucleic Acids Res	2024	https://conductscience.org/library/papers/39119921	PubMed
38,407,477	Novel mechanisms of diversity generation in Acinetobacter baumannii resistance islands driven by Tn7-like elements.	Nucleic Acids Res	2024	https://conductscience.org/library/papers/38407477	PubMed
37,283,088	Widespread CRISPR-derived RNA regulatory elements in CRISPR-Cas systems.	Nucleic Acids Res	2023	https://conductscience.org/library/papers/37283088	PubMed
36,537,206	Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria.	Nucleic Acids Res	2023	https://conductscience.org/library/papers/36537206	PubMed
35,208,691	inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes.	Microorganisms	2022	https://conductscience.org/library/papers/35208691	PubMed
34,125,584	Repurposing CRISPR-Cas Systems as Genetic Tools for the Enterobacteriales.	EcoSal Plus	2021	https://conductscience.org/library/papers/34125584	PubMed
28,096,446	Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer.	J Bacteriol	2017	https://conductscience.org/library/papers/28096446	PubMed
27,907,029	Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions.	PLoS One	2016	https://conductscience.org/library/papers/27907029	PubMed
26,162,088	Novel recA-Independent Horizontal Gene Transfer in Escherichia coli K-12.	PLoS One	2015	https://conductscience.org/library/papers/26162088	PubMed

1 to 10 of 12

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Genes1

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

Gene	NCBI Gene ID	Linked by	In Genes
GLB1	2,720	PubTator's text mining	https://conductscience.org/library/genes/2720

Source NCBI Gene

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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