Paper · 29482575

i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.

Ohtsuka M and 9 others. Genome Biol, 2018. Cited by 166 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Papers, record 29482575. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/29482575

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.; PubMed
Journal: Genome Biol; PubMed
Year: 2018; PubMed
Publication types: Journal Article, Research Support, Non-U.S. Gov't; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/29482575/; PubMed
DOI: https://doi.org/10.1186/s13059-018-1400-x; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC5828090/; PubMed
The journal: https://conductscience.org/library/journal-index/100960660; PubMed
Citations, by iCite: 166; iCite
Relative citation ratio, by iCite: 5.1; iCite
Revised in PubMed: 2026-01-27; PubMed

Authors10

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename	ORCID iD
1	Ohtsuka M	Masato	https://orcid.org/0000-0002-6952-4238
2	Sato M	Masahiro
3	Miura H	Hiromi
4	Takabayashi S	Shuji
5	Matsuyama M	Makoto
6	Koyano T	Takayuki
7	Arifin N	Naomi
8	Nakamura S	Shingo
9	Wada K	Kenta
10	Gurumurthy CB	Channabasavaiah B

Source PubMed

Topics18

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Bacterial Proteins	Major topic
CRISPR-Cas Systems	Major topic
Endonucleases	Major topic
Gene Editing	Major topic
Animals
CRISPR-Associated Proteins
Electroporation
Female
Forkhead Transcription Factors
Gene Knock-In Techniques
Mice, Inbred Strains
Mice, Knockout
Mice, Transgenic
Microinjections
Ovary
Pregnancy
Sequence Deletion
Endodeoxyribonucleases

Source PubMed

Grants5

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes it	Agency	Country	Stated by
16K18821	Japan Society for the Promotion of Science	International	PubMed
15K14371	Japan Society for the Promotion of Science	International	PubMed
16H05049	Japan Society for the Promotion of Science	International	PubMed
2014	Tokai University School of Medicine Research Aid	International	PubMed
2016-2017	Tokai University School of Medicine Project Research	International	PubMed

Source PubMed

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections1

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

Kind	PMID	Title	In Papers
Comment in	29,490,686	Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology.	https://conductscience.org/library/papers/29490686

Source PubMed

References29

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	28,351,759	Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.	Methods	2017	https://conductscience.org/library/papers/28351759	PubMed
2	26,422,227	Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.	Cell	2015	https://conductscience.org/library/papers/26422227	PubMed
3	27,158,268	Roles for retrotransposon insertions in human disease.	Mob DNA	2016	https://conductscience.org/library/papers/27158268	PubMed
4	23,643,243	One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.	Cell	2013	https://conductscience.org/library/papers/23643243	PubMed
5	27,384,229	CRISPR: a versatile tool for both forward and reverse genetics research.	Hum Genet	2016	https://conductscience.org/library/papers/27384229	PubMed
6	22,002,806	A deletion in a cis element of Foxe3 causes cataracts and microphthalmia in rct mice.	Mamm Genome	2011	https://conductscience.org/library/papers/22002806	PubMed
7	11,476,355	Culture of preimplantation embryos and its long-term effects on gene expression and phenotype.	Hum Reprod Update	2001	https://conductscience.org/library/papers/11476355	PubMed
8	26,137,492	Precision Medicine for Continuing Phenotype Expansion of Human Genetic Diseases.	Biomed Res Int	2015	https://conductscience.org/library/papers/26137492	PubMed
9	16,199,865	Severe defects in proliferation and differentiation of lens cells in Foxe3 null mice.	Mol Cell Biol	2005	https://conductscience.org/library/papers/16199865	PubMed
10	25,819,794	Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.	Genetics	2015	https://conductscience.org/library/papers/25819794	PubMed

1 to 10 of 29

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Cited by119

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 166 citations.

PMID	Title	Journal	Year	In Papers	Source
42,661,411	Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression.	Biotechnol J	2026	https://conductscience.org/library/papers/42661411	PubMed
42,536,701	Branched-chain amino acid transaminase 1-mediated pathway promotes proline-dependent collagen production in cardiac myofibroblasts.	J Clin Invest	2026	https://conductscience.org/library/papers/42536701	PubMed
42,290,195	Clarifying Frizzled 2 function in development through genetically validated mouse models.	Dis Model Mech	2026	https://conductscience.org/library/papers/42290195	PubMed
42,201,943	A portable, ultra-low cost, open-source, pedal-controlled microinjector for laboratory use.	PLoS One	2026	https://conductscience.org/library/papers/42201943	PubMed
42,084,361	Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.	mBio	2026	https://conductscience.org/library/papers/42084361	PubMed
41,972,436	PSMA8-Containing 20S Proteasome Regulates Spermiogenesis and Male Fertility.	Adv Sci (Weinh)	2026	https://conductscience.org/library/papers/41972436	PubMed
41,813,164	Effect of post-weaning diet on hibernation occurrence in Syrian hamsters.	Exp Anim	2026	https://conductscience.org/library/papers/41813164	PubMed
41,727,189	Reduced Akr1b7 signaling drives ovarian aging and reproductive dysfunction.	iScience	2026	https://conductscience.org/library/papers/41727189	PubMed
41,709,464	Non-invasive ovulation tracking enables genetic engineering in wild rodents.	Cell Rep Methods	2026	https://conductscience.org/library/papers/41709464	PubMed
41,634,336	DGCR8 regulates multiple processes of transcription coupled nucleotide excision repair.	Sci Rep	2026	https://conductscience.org/library/papers/41634336	PubMed

1 to 10 of 119

See all 119 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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