Paper · 3313383

Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.

Aebersold RH and 4 others. Proc Natl Acad Sci U S A, 1987. Cited by 787 papers, by iCite.

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ConductScience Library. Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Papers, record 3313383. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/3313383

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title: Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.; PubMed
Journal: Proc Natl Acad Sci U S A; PubMed
Year: 1987; PubMed
Publication types: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.; PubMed
PubMed: https://pubmed.ncbi.nlm.nih.gov/3313383/; PubMed
DOI: https://doi.org/10.1073/pnas.84.20.6970; PubMed
PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC299210/; PubMed
The journal: https://conductscience.org/library/journal-index/7505876; PubMed
Citations, by iCite: 787; iCite
Relative citation ratio, by iCite: 17.7; iCite
Revised in PubMed: 2019-05-01; PubMed

Authors5

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

Order	Author	Forename
1	Aebersold RH	R H
2	Leavitt J	J
3	Saavedra RA	R A
4	Hood LE	L E
5	Kent SB	S B

Source PubMed

Topics9

NLM's MeSH indexing of the paper, its major topics first.

MeSH term	Major
Amino Acid Sequence	Major topic
Electrophoresis, Polyacrylamide Gel	Major topic
Peptide Fragments	Major topic
Proteins	Major topic
Chromatography, High Pressure Liquid
Collodion
Membranes, Artificial
Peptide Hydrolases
Peptide Mapping

Source PubMed

Grants2

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes it	Agency	Country	NIH project	Stated by	Source
CA 32911	NCI NIH HHS	United States	P30CA032911	PubMed and NIH	NIH RePORTER
CA 34763	NCI NIH HHS	United States	R01CA034763	PubMed and NIH	NIH RePORTER

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References19

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

Order	PMID	Title	Journal	Year	In Papers	Source
1	5,432,063	Cleavage of structural proteins during the assembly of the head of bacteriophage T4.	Nature	1970	https://conductscience.org/library/papers/5432063	PubMed
2	236,308	High resolution two-dimensional electrophoresis of proteins.	J Biol Chem	1975	https://conductscience.org/library/papers/236308	PubMed
3	241,077	An octamer of histones in chromatin and free in solution.	Proc Natl Acad Sci U S A	1975	https://conductscience.org/library/papers/241077	PubMed
4	626,405	Polyquarternary amines prevent peptide loss from sequenators.	Anal Biochem	1978	https://conductscience.org/library/papers/626405	PubMed
5	388,439	Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.	Proc Natl Acad Sci U S A	1979	https://conductscience.org/library/papers/388439	PubMed
6	7,263,636	A gas-liquid solid phase peptide and protein sequenator.	J Biol Chem	1981	https://conductscience.org/library/papers/7263636	PubMed
7	6,298,222	The amino acid sequence of the beta-subunit of ATP synthase from bovine heart mitochondria.	J Biol Chem	1983	https://conductscience.org/library/papers/6298222	PubMed
8	6,855,576	Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.	Methods Enzymol	1983	https://conductscience.org/library/papers/6855576	PubMed
9	6,855,596	Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography.	Methods Enzymol	1983	https://conductscience.org/library/papers/6855596	PubMed
10	2,983,222	Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.	Nature	1985	https://conductscience.org/library/papers/2983222	PubMed

1 to 10 of 19

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Cited by210

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 787 citations.

PMID	Title	Journal	Year	In Papers	Source
42,129,069	Proteomic Technology.	Adv Exp Med Biol	2026	https://conductscience.org/library/papers/42129069	PubMed
42,048,638	A Selective History of Protein Sequencing and the Impact of Donald F. Hunt on Protein Sequencing by Tandem Mass Spectrometry.	J Proteome Res	2026	https://conductscience.org/library/papers/42048638	PubMed
41,904,654	Comparison of In-Gel and SP3 Based Sample Preparation Protocols for LC-MS/MS Based Proteomics.	Proteomics	2026	https://conductscience.org/library/papers/41904654	PubMed
40,319,309	Recent advances in omics and the integration of multi-omics in osteoarthritis research.	Arthritis Res Ther	2025	https://conductscience.org/library/papers/40319309	PubMed
39,531,497	Ultradeep O-GlcNAc proteomics reveals widespread O-GlcNAcylation on tyrosine residues of proteins.	Proc Natl Acad Sci U S A	2024	https://conductscience.org/library/papers/39531497	PubMed
36,959,352	Global detection of human variants and isoforms by deep proteome sequencing.	Nat Biotechnol	2023	https://conductscience.org/library/papers/36959352	PubMed
33,950,482	The Whereabouts of 2D Gels in Quantitative Proteomics.	Methods Mol Biol	2021	https://conductscience.org/library/papers/33950482	PubMed
31,556,486	An integrated workflow for crosslinking mass spectrometry.	Mol Syst Biol	2019	https://conductscience.org/library/papers/31556486	PubMed
31,402,847	Purification and molecular characterization of NP185, a neuronal-specific and synapse-enriched clathrin assembly polypeptide.	Bioquim Patol Clin	1998	https://conductscience.org/library/papers/31402847	PubMed
28,993,761	PCR and Omics Based Techniques to Study the Diversity, Ecology and Biology of Anaerobic Fungi: Insights, Challenges and Opportunities.	Front Microbiol	2017	https://conductscience.org/library/papers/28993761	PubMed

1 to 10 of 210

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Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed: 2026-11-06; PubMed
iCite: 2026-11-05; iCite
PMC's ID file: 2026-11-05; PMC's ID file
NIH RePORTER's grant and paper links: 2026-11-01; NIH RePORTER's grant and paper links
Trials: 2026-11-12
Gene search: 2026-11-11
Pipelines: 2026-09-18

Sources 11

ChEMBL, through Open TargetsCC BY-SA 3.0
ClinicalTrials.govNo license stated
HGNCCC0
iCiteCC BY 4.0
NCBI GenePublic domain (US government)
NIH RePORTER's grant and paper linksPublic domain
Open TargetsCC0 1.0
PMC's ID filePublic domain (US government)
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0

Each source's terms

ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
ClinicalTrials.govnone stated
HGNCCC0: https://www.genenames.org/about/license/
iCiteCC BY 4.0
NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
PubMedNLM's terms
PubTator 3Public domain
UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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