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Paper · 388356

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Birnboim HC and 1 other. Nucleic Acids Res, 1979. Cited by 12,298 papers, by iCite.

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ConductScience Library. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Papers, record 388356. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/388356

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
PubMed
Journal
Nucleic Acids Res
PubMed
Year
1979
PubMed
Publication types
Journal Article
PubMed
Citations, by iCite
12,298
iCite
Relative citation ratio, by iCite
352
iCite
Revised in PubMed
2024-01-09
PubMed

Authors2

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Birnboim HCH C
2Doly JJ

Source PubMed

Topics12

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
Cloning, MolecularMajor topic
DNA, RecombinantMajor topic
PlasmidsMajor topic
Animals
Chromosomes, Bacterial
DNA Restriction Enzymes
DNA, Circular
Escherichia coli
Methods
Mice
Molecular Weight
Transformation, Bacterial

Source PubMed

Grants0

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

None in this release.

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References17

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
3360,212Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.Proc Natl Acad Sci U S A1978https://conductscience.org/library/papers/360212PubMed
411,708Isolation of covalently closed circular DNA of high molecular weight from bacteria.Anal Biochem1976https://conductscience.org/library/papers/11708PubMed
5107,540A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.Plasmid1978https://conductscience.org/library/papers/107540PubMed
6363,426Rapid purification of plasmid DNAs by hydroxyapatite chromatography.Eur J Biochem1978https://conductscience.org/library/papers/363426PubMed
7344,137Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene1977https://conductscience.org/library/papers/344137PubMed
8331,257Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.Nucleic Acids Res1977https://conductscience.org/library/papers/331257PubMed
9318,764Plasmid detection and sizing in single colony lysates.Science1977https://conductscience.org/library/papers/318764PubMed
10318,763Novel screening procedure for recombinant plasmids.Science1977https://conductscience.org/library/papers/318763PubMed
11797,459Recombination between bacterial plasmids leading to the formation of plasmid multimers.Cell1976https://conductscience.org/library/papers/797459PubMed
124,336,693Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.J Bacteriol1972https://conductscience.org/library/papers/4336693PubMed

1 to 10 of 17

See all 17 references

Cited by5,626

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 12,298 citations.

PMIDTitleJournalYearIn PapersSource
42,980,930Plasmid-mediated dissemination of antimicrobial resistance among multidrug-resistant bacteria isolated from food-contact surfaces in university cafeterias.Mol Biol Rep2026https://conductscience.org/library/papers/42980930PubMed
42,704,564Role of estrogen-related receptor alpha in osteogenic differentiation.Hum Cell2026https://conductscience.org/library/papers/42704564PubMed
42,681,050The Use of Yeast Two-Hybrid Analysis to Study Protein-Protein Interactions for Ustilago maydis.Methods Mol Biol2026https://conductscience.org/library/papers/42681050PubMed
42,544,871Phytochemical Characterization and Biological Activities of Anthocleista nobilis Bark Extracts Against Multidrug-Resistant Uropathogens.Chem Biodivers2026https://conductscience.org/library/papers/42544871PubMed
42,542,460Rocaglates and pateamines target the DEAD-box RNA helicase eIF4A from Schistosoma mansoni and demonstrate anti-schistosomal activity in vitro.Sci Rep2026https://conductscience.org/library/papers/42542460PubMed
42,429,570A UG5 reverse transcriptase-nitrilase antiviral module confers phage immunity in the plant symbiont Sinorhizobium meliloti.Microbiol Spectr2026https://conductscience.org/library/papers/42429570PubMed
42,369,543Active biosynthesis of gold nanoparticles mediated by obligate methylotrophic bacteria Methylophilus sp.Front Microbiol2026https://conductscience.org/library/papers/42369543PubMed
42,167,936Identification of proteins interacting with the N-terminal half of endoribonuclease RNase E in Escherichia coli.RNA Biol2026https://conductscience.org/library/papers/42167936PubMed
41,908,593Coexistence and Genetic Location of Aminoglycoside-Modifying Enzyme Genes in Clinical Escherichia coli Isolates from a Tertiary Hospital in Ouagadougou.Infect Drug Resist2026https://conductscience.org/library/papers/41908593PubMed
41,900,143
The Difluoroboranyl-Fluoroquinolone Derivative "7a" Inhibits Bacterial DNA Gyrase and Exhibits Potent Activity Against Ciprofloxacin-Resistant S. aureus In Vitro and In Vivo Using an Acute Pneumonia Model.
Molecules2026https://conductscience.org/library/papers/41900143PubMed

1 to 10 of 5,626

See all 5,626 cited by

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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