Paper · 40975871
Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.
Washer SJ and 4 others. STAR Protoc, 2025. Cited by 1 paper, by iCite.
11 sourcespapers-2026-11-12-0.1.0
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ConductScience Library. Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia. Papers, record 40975871. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/40975871
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Paper
PubMed's record of the paper, with iCite's citation figures. No abstract is shown.
- Title
- Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.
- PubMed
Topics8
NLM's MeSH indexing of the paper, its major topics first.
| MeSH term | Major |
|---|---|
| Induced Pluripotent Stem Cells | Major topic |
| Microglia | Major topic |
| CRISPR-Cas Systems | Major topic |
| Gene Knockout Techniques | Major topic |
| Flow Cytometry | Major topic |
| Humans | |
| Lentivirus | |
| Phagocytosis |
Source PubMed
Grants0
Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.
None in this release.
Trials0
The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.
None in this release.
Comments and corrections0
PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.
None in this release.
References14
The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.
| Order | PMID | Title | Journal | Year | In Papers | Source |
|---|---|---|---|---|---|---|
| 1 | 35,379,992 | New insights into the genetic etiology of Alzheimer's disease and related dementias. | Nat Genet | 2022 | https://conductscience.org/library/papers/35379992 | PubMed |
| 2 | 41,353,404 | A next-generation dual guide CRISPR system for genetic interaction library screening. | Nat Commun | 2025 | https://conductscience.org/library/papers/41353404 | PubMed |
| 3 | 36,376,339 | Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSC to microglia. | Sci Rep | 2022 | https://conductscience.org/library/papers/36376339 | PubMed |
| 4 | 29,224,076 | Next-Generation Sequencing of Genome-Wide CRISPR Screens. | Methods Mol Biol | 2018 | https://conductscience.org/library/papers/29224076 | PubMed |
| 5 | 27,760,321 | A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. | Cell Rep | 2016 | https://conductscience.org/library/papers/27760321 | PubMed |
| 6 | 36,459,969 | A reference human induced pluripotent stem cell line for large-scale collaborative studies. | Cell Stem Cell | 2022 | https://conductscience.org/library/papers/36459969 | PubMed |
| 7 | 33,427,296 | The microglial component of amyotrophic lateral sclerosis. | Brain | 2020 | https://conductscience.org/library/papers/33427296 | PubMed |
| 8 | 38,401,123 | Optimized protocol for CRISPR knockout of human iPSC-derived macrophages. | STAR Protoc | 2024 | https://conductscience.org/library/papers/38401123 | PubMed |
| 9 | 33,589,840 | Genome-wide meta-analysis, fine-mapping and integrative prioritization implicate new Alzheimer's disease risk genes. | Nat Genet | 2021 | https://conductscience.org/library/papers/33589840 | PubMed |
| 10 | 25,075,903 | Improved vectors and genome-wide libraries for CRISPR screening. | Nat Methods | 2014 | https://conductscience.org/library/papers/25075903 | PubMed |
1 to 10 of 14
See all 14 referencesCited by1
The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 1 citation.
| PMID | Title | Journal | Year | In Papers |
|---|---|---|---|---|
| 41,943,483 | Tracking tau and cellular responses in human iPSC-microglia: from uptake to seedable secretion, including in extracellular vesicles. | Alzheimers Dement | 2026 | https://conductscience.org/library/papers/41943483 |
Source PubMed
Genes0
The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.
None in this release.
Drugs0
The drugs whose trials, in the Pipelines release, list the paper.
None in this release.
Data0
The data bank entries PubMed's record names. Trial numbers are under Trials.
None in this release.
Data dates
The date of each source this release read.
- Trials
- 2026-11-12
- Gene search
- 2026-11-11
- Pipelines
- 2026-09-18
Sources 11
- ChEMBL, through Open TargetsCC BY-SA 3.0
- ClinicalTrials.govNo license stated
- HGNCCC0
- iCiteCC BY 4.0
- NCBI GenePublic domain (US government)
- NIH RePORTER's grant and paper linksPublic domain
- Open TargetsCC0 1.0
- PMC's ID filePublic domain (US government)
- PubMedNLM's terms
- PubTator 3Public domain
- UniProtCC BY 4.0
Each source's terms
- ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
- ClinicalTrials.govnone stated
- HGNCCC0: https://www.genenames.org/about/license/
- iCiteCC BY 4.0
- NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
- NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
- Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
- PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
- PubMedNLM's terms
- PubTator 3Public domain
- UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license
ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.
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