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Paper · 41353404

A next-generation dual guide CRISPR system for genetic interaction library screening.

Burgold T and 12 others. Nat Commun, 2025. Cited by 6 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. A next-generation dual guide CRISPR system for genetic interaction library screening. Papers, record 41353404. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/41353404

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
A next-generation dual guide CRISPR system for genetic interaction library screening.
PubMed
Journal
Nat Commun
PubMed
Year
2025
PubMed
Publication types
Journal Article, Research Support, Non-U.S. Gov't
PubMed
Citations, by iCite
6
iCite
Relative citation ratio, by iCite
1.32, provisional
iCite
Revised in PubMed
2026-10-20
PubMed

Authors13

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Burgold TThomashttps://orcid.org/0009-0006-8324-8934
2Karakoc EEmre
3Gonçalves EEmanuelhttps://orcid.org/0000-0002-9967-5205
4Barrio-Hernandez IInigo
5Dwane LLisa
6Silva RRominahttps://orcid.org/0000-0001-5429-0152
7Souster EEmily
8Sharma MMamta
9Beck AAlexandra
10Koh GCCGene Ching Chiekhttps://orcid.org/0000-0002-3840-4954

1 to 10 of 13Source PubMed

See all 13 authors

Topics10

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
CRISPR-Cas SystemsMajor topic
RNA, Guide, CRISPR-Cas SystemsMajor topic
Gene LibraryMajor topic
Humans
Cell Line, Tumor
Streptococcus pyogenes
Colorectal Neoplasms
CRISPR-Associated Protein 9
Clustered Regularly Interspaced Short Palindromic Repeats
RNA, Transfer

Source PubMed

Grants2

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated by
Wellcome TrustUnited KingdomPubMed
220540/Z/20/AWellcome Trust (Wellcome)PubMed

Source PubMed

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References42

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
129,799,876Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens.PLoS One2018https://conductscience.org/library/papers/29799876PubMed
226,422,227Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.Cell2015https://conductscience.org/library/papers/26422227PubMed
332,231,336Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing.Nat Biotechnol2020https://conductscience.org/library/papers/32231336PubMed
429,211,736Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression.PLoS One2017https://conductscience.org/library/papers/29211736PubMed
530,971,826Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.Nature2019https://conductscience.org/library/papers/30971826PubMed
630,971,823WRN helicase is a synthetic lethal target in microsatellite unstable cancers.Nature2019https://conductscience.org/library/papers/30971823PubMed
735,589,728Multiplex base- and prime-editing with drive-and-process CRISPR arrays.Nat Commun2022https://conductscience.org/library/papers/35589728PubMed
829,457,792On the design of CRISPR-based single-cell molecular screens.Nat Methods2018https://conductscience.org/library/papers/29457792PubMed
931,634,902Search-and-replace genome editing without double-strand breaks or donor DNA.Nature2019https://conductscience.org/library/papers/31634902PubMed
1031,422,865CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.Neuron2019https://conductscience.org/library/papers/31422865PubMed

1 to 10 of 42

See all 42 references

Cited by8

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 6 citations.

PMIDTitleJournalYearIn PapersSource
42,049,019Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.Cell2026https://conductscience.org/library/papers/42049019PubMed
41,887,225Balancing off-target and on-target considerations for optimized CRISPR-Cas9 knockout library design.Cell Genom2026https://conductscience.org/library/papers/41887225PubMed
41,542,642Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens.bioRxiv2026https://conductscience.org/library/papers/41542642PubMed
41,446,269Functional modules predict cancer-relevant genetic interactions in mammalian cells.bioRxiv2025https://conductscience.org/library/papers/41446269PubMed
40,975,871Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.STAR Protoc2025https://conductscience.org/library/papers/40975871PubMed
40,737,092Altered mRNA transport and local translation in i3Neurons with RNA-binding protein knockdown.Nucleic Acids Res2025https://conductscience.org/library/papers/40737092PubMed
40,631,273A global genetic interaction map of a human cell reveals conserved principles of genetic networks.bioRxiv2025https://conductscience.org/library/papers/40631273PubMed
39,386,562Altered mRNA transport and local translation in iNeurons with RNA binding protein knockdown.bioRxiv2024https://conductscience.org/library/papers/39386562PubMed

Genes0

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

None in this release.

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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