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Paper · 23934178

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.

Pattanayak V and 5 others. Nat Biotechnol, 2013. Cited by 1,133 papers, by iCite.

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ConductScience Library. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Papers, record 23934178. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/23934178

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.
PubMed
Journal
Nat Biotechnol
PubMed
Year
2013
PubMed
Publication types
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
Citations, by iCite
1,133
iCite
Relative citation ratio, by iCite
28.5
iCite
Revised in PubMed
2024-01-04
PubMed

Authors6

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Pattanayak VVikram
2Lin SSteven
3Guilinger JPJohn P
4Ma EEnbo
5Doudna JAJennifer A
6Liu DRDavid R

Source PubMed

Topics12

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
EndonucleasesMajor topic
Genetic EngineeringMajor topic
High-Throughput Nucleotide SequencingMajor topic
Sequence Analysis, DNAMajor topic
Bacterial Proteins
DNA
Genome
Genomics
HEK293 Cells
Humans
Streptococcus pyogenes
RNA, Small Untranslated

Source PubMed

Grants5

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated bySource
Howard Hughes Medical InstituteUnited StatesPubMedPubMed
T32 GM007753NIGMS NIH HHSUnited StatesT32GM007753PubMed and NIHNIH RePORTER
R01GM073794-05NIGMS NIH HHSUnited StatesR01GM073794PubMed and NIHNIH RePORTER
R01 GM073794NIGMS NIH HHSUnited StatesR01GM073794PubMed and NIHNIH RePORTER
T32GM007753NIGMS NIH HHSUnited StatesT32GM007753PubMed and NIHNIH RePORTER

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections1

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

KindPMIDTitleIn Papers
Comment in24,022,156Staying on target with CRISPR-Cas.https://conductscience.org/library/papers/24022156

Source PubMed

References21

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
123,360,964Efficient genome editing in zebrafish using a CRISPR-Cas system.Nat Biotechnol2013https://conductscience.org/library/papers/23360964PubMed
223,360,965RNA-guided editing of bacterial genomes using CRISPR-Cas systems.Nat Biotechnol2013https://conductscience.org/library/papers/23360965PubMed
319,540,188Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.Cell Stem Cell2009https://conductscience.org/library/papers/19540188PubMed
421,151,125Targeted integration in rat and mouse embryos with zinc-finger nucleases.Nat Biotechnol2011https://conductscience.org/library/papers/21151125PubMed
523,452,860Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.Cell2013https://conductscience.org/library/papers/23452860PubMed
621,813,460The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli.Nucleic Acids Res2011https://conductscience.org/library/papers/21813460PubMed
722,745,249A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.Science2012https://conductscience.org/library/papers/22745249PubMed
821,822,241Targeted gene disruption in somatic zebrafish cells using engineered TALENs.Nat Biotechnol2011https://conductscience.org/library/papers/21822241PubMed
918,500,337Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases.Nat Biotechnol2008https://conductscience.org/library/papers/18500337PubMed
1023,287,718Multiplex genome engineering using CRISPR/Cas systems.Science2013https://conductscience.org/library/papers/23287718PubMed

1 to 10 of 21

See all 21 references

Cited by820

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 1,133 citations.

PMIDTitleJournalYearIn PapersSource
42,910,235Human Induced Pluripotent Stem Cells in Rare Neurological Disorders: Opportunities and Challenges for Disease Modeling and Drug Discovery.Stem Cells Int2026https://conductscience.org/library/papers/42910235PubMed
42,877,411Gene therapy for non-obstructive azoospermia: therapeutic avenues and translational pathway to clinical application.Front Genome Ed2026https://conductscience.org/library/papers/42877411PubMed
42,742,174Highly efficient base editing at PCSK9 and normal human embryo development.Nature2026https://conductscience.org/library/papers/42742174PubMed
42,694,036Three hydrophobic surface binding a proteins link surface hydrophobicity to sporulation, biofilm formation, and host interaction in Mucor lusitanicus.Front Cell Infect Microbiol2026https://conductscience.org/library/papers/42694036PubMed
42,649,204Single-cell and in vivo profiling reveal heterogeneous and organ-specific CRISPR-Cas9 off-target and translocation outcomes.Nat Commun2026https://conductscience.org/library/papers/42649204PubMed
42,620,363Genomic repeats for single-cell molecular recording.bioRxiv2026https://conductscience.org/library/papers/42620363PubMed
42,333,535SNIPSNP: precision design of CRISPR/Cas9 knock-in reagents for variant correction and disease modeling.Nucleic Acids Res2026https://conductscience.org/library/papers/42333535PubMed
42,310,027iSCORE-PD: an isogenic stem cell collection to research Parkinson's disease.Nat Commun2026https://conductscience.org/library/papers/42310027PubMed
42,273,291Design and validation of an inducible and curable EvolvR system for directed evolution in Corynebacterium glutamicum.Front Bioeng Biotechnol2026https://conductscience.org/library/papers/42273291PubMed
42,170,219Off-target detection of CRISPR-Cas9 nuclease in vitro with CROFT-Seq.Synth Biol (Oxf)2026https://conductscience.org/library/papers/42170219PubMed

1 to 10 of 820

See all 820 cited by

Genes6

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

GeneNCBI Gene IDLinked byIn GenesSource
CDK81,024PubTator's text mininghttps://conductscience.org/library/genes/1024NCBI Gene
CLTA1,211PubTator's text mininghttps://conductscience.org/library/genes/1211NCBI Gene
NEB4,703PubTator's text mininghttps://conductscience.org/library/genes/4703NCBI Gene
NR1H49,971PubTator's text mininghttps://conductscience.org/library/genes/9971NCBI Gene
SEPHS222,928PubTator's text mininghttps://conductscience.org/library/genes/22928NCBI Gene
SLC9A26,549PubTator's text mininghttps://conductscience.org/library/genes/6549NCBI Gene

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data0

The data bank entries PubMed's record names. Trial numbers are under Trials.

None in this release.

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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