Paper · 32217751
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT and 3 others. Science, 2020. Cited by 987 papers, by iCite.
11 sourcespapers-2026-11-12-0.1.0
Cite this record
ConductScience Library. Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Papers, record 32217751. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/32217751
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Paper
PubMed's record of the paper, with iCite's citation figures. No abstract is shown.
- Publication types
- Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
- PubMed
Topics10
NLM's MeSH indexing of the paper, its major topics first.
| MeSH term | Major |
|---|---|
| CRISPR-Associated Protein 9 | Major topic |
| CRISPR-Cas Systems | Major topic |
| Gene Editing | Major topic |
| Gene Targeting | Major topic |
| Genetic Predisposition to Disease | Major topic |
| HEK293 Cells | |
| Humans | |
| Mutagenesis | |
| Protein Domains | |
| Substrate Specificity |
Source PubMed
Grants2
Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.
| Grant, as the record writes it | Agency | Country | NIH project | Stated by | Source |
|---|---|---|---|---|---|
| P01 HL142494 | NHLBI NIH HHS | United States | P01HL142494 | PubMed and NIH | NIH RePORTER |
| R00 CA218870 | NCI NIH HHS | United States | R00CA218870 | PubMed and NIH | NIH RePORTER |
Trials0
The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.
None in this release.
Comments and corrections3
PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.
| Kind | PMID | Title | In Papers | Source |
|---|---|---|---|---|
| Comment in | 32,416,935 | Rewired Cas9s with Minimal Sequence Constraints. | https://conductscience.org/library/papers/32416935 | PubMed |
| Comment in | 32,456,805 | SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power. | https://conductscience.org/library/papers/32456805 | PubMed |
| Comment in | 32,499,620 | PAM-less is more. | https://conductscience.org/library/papers/32499620 | PubMed |
References44
The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.
| Order | PMID | Title | Journal | Year | In Papers | Source |
|---|---|---|---|---|---|---|
| 1 | 28,581,492 | Engineered Cpf1 variants with altered PAM specificities. | Nat Biotechnol | 2017 | https://conductscience.org/library/papers/28581492 | PubMed |
| 2 | 18,065,545 | Phage response to CRISPR-encoded resistance in Streptococcus thermophilus. | J Bacteriol | 2008 | https://conductscience.org/library/papers/18065545 | PubMed |
| 3 | 25,513,782 | GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. | Nat Biotechnol | 2015 | https://conductscience.org/library/papers/25513782 | PubMed |
| 4 | 26,524,662 | Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. | Nat Biotechnol | 2015 | https://conductscience.org/library/papers/26524662 | PubMed |
| 5 | 29,512,652 | Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. | Nature | 2018 | https://conductscience.org/library/papers/29512652 | PubMed |
| 6 | 29,160,308 | Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. | Nature | 2017 | https://conductscience.org/library/papers/29160308 | PubMed |
| 7 | 30,323,312 | Base editing: precision chemistry on the genome and transcriptome of living cells. | Nat Rev Genet | 2018 | https://conductscience.org/library/papers/30323312 | PubMed |
| 8 | 26,628,643 | Rationally engineered Cas9 nucleases with improved specificity. | Science | 2016 | https://conductscience.org/library/papers/26628643 | PubMed |
| 9 | 24,076,762 | Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. | Nat Methods | 2013 | https://conductscience.org/library/papers/24076762 | PubMed |
| 10 | 20,072,129 | Self versus non-self discrimination during CRISPR RNA-directed immunity. | Nature | 2010 | https://conductscience.org/library/papers/20072129 | PubMed |
1 to 10 of 44
See all 44 referencesCited by771
The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 987 citations.
| PMID | Title | Journal | Year | In Papers | Source |
|---|---|---|---|---|---|
| 42,966,963 | Regeneration, genetic transformation, and gene editing in Vaccinium species: current progress and future directions. | Hortic Res | 2026 | https://conductscience.org/library/papers/42966963 | PubMed |
| 42,957,916 | Research progress of gene correction for genetic diseases via in vivo prime editing. | Mol Genet Genomics | 2026 | https://conductscience.org/library/papers/42957916 | PubMed |
| 42,953,289 | Reshaping the scissors: From directed evolution to AI-driven design for enhanced Cas performance. | Biodes Res | 2026 | https://conductscience.org/library/papers/42953289 | PubMed |
| 42,920,906 | Minimal indel-inducing and window-adjustable base editing using inactive Cas12b effectors. | Mol Ther Nucleic Acids | 2026 | https://conductscience.org/library/papers/42920906 | PubMed |
| 42,911,482 | Role of Chromatin-Associated RNAs in Eye Physiology and Pathology. | Methods Mol Biol | 2027 | https://conductscience.org/library/papers/42911482 | PubMed |
| 42,909,796 | Base editing-mediated exon-specific knockout of glutamine synthetase generates a high-productivity CHO cell line for antibody manufacturing. | Bioresour Bioprocess | 2026 | https://conductscience.org/library/papers/42909796 | PubMed |
| 42,891,351 | Strategies and mechanisms of precision genome engineering: From gene editing to genome writing. | IMetaOmics | 2026 | https://conductscience.org/library/papers/42891351 | PubMed |
| 42,888,260 | Translating base editors: a decade of progress from the bench to the clinic. | Nat Rev Drug Discov | 2026 | https://conductscience.org/library/papers/42888260 | PubMed |
| 42,840,375 | Engineering TnpB as a compact RNA-guided genome editor from molecular constraints to design principles. | Front Genome Ed | 2026 | https://conductscience.org/library/papers/42840375 | PubMed |
| 42,835,755 | Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Beta vulgaris L.). | Front Plant Sci | 2026 | https://conductscience.org/library/papers/42835755 | PubMed |
1 to 10 of 771
See all 771 cited byGenes7
The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.
| Gene | NCBI Gene ID | Linked by | In Genes | Source |
|---|---|---|---|---|
| CFH | 3,075 | PubTator's text mining | https://conductscience.org/library/genes/3075 | NCBI Gene |
| CSPG5 | 10,675 | PubTator's text mining | https://conductscience.org/library/genes/10675 | NCBI Gene |
| MSTN | 2,660 | PubTator's text mining | https://conductscience.org/library/genes/2660 | NCBI Gene |
| NEO1 | 4,756 | PubTator's text mining | https://conductscience.org/library/genes/4756 | NCBI Gene |
| NRN1 | 51,299 | PubTator's text mining | https://conductscience.org/library/genes/51299 | NCBI Gene |
| SOST | 50,964 | PubTator's text mining | https://conductscience.org/library/genes/50964 | NCBI Gene |
| SPG16 | 57,760 | PubTator's text mining | https://conductscience.org/library/genes/57760 | NCBI Gene |
Drugs0
The drugs whose trials, in the Pipelines release, list the paper.
None in this release.
Data0
The data bank entries PubMed's record names. Trial numbers are under Trials.
None in this release.
Data dates
The date of each source this release read.
- Trials
- 2026-11-12
- Gene search
- 2026-11-11
- Pipelines
- 2026-09-18
Sources 11
- ChEMBL, through Open TargetsCC BY-SA 3.0
- ClinicalTrials.govNo license stated
- HGNCCC0
- iCiteCC BY 4.0
- NCBI GenePublic domain (US government)
- NIH RePORTER's grant and paper linksPublic domain
- Open TargetsCC0 1.0
- PMC's ID filePublic domain (US government)
- PubMedNLM's terms
- PubTator 3Public domain
- UniProtCC BY 4.0
Each source's terms
- ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
- ClinicalTrials.govnone stated
- HGNCCC0: https://www.genenames.org/about/license/
- iCiteCC BY 4.0
- NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
- NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
- Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
- PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
- PubMedNLM's terms
- PubTator 3Public domain
- UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license
ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.
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