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Paper · 24463574

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Fu Y and 4 others. Nat Biotechnol, 2014. Cited by 1,526 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Papers, record 24463574. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/24463574

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Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.
PubMed
Journal
Nat Biotechnol
PubMed
Year
2014
PubMed
Publication types
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
Citations, by iCite
1,526
iCite
Relative citation ratio, by iCite
41
iCite
Revised in PubMed
2024-01-04
PubMed

Authors5

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Fu YYanfang
2Sander JDJeffry D
3Reyon DDeepak
4Cascio VMVincent M
5Joung JKJ Keith

Source PubMed

Topics8

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
Bacterial ProteinsMajor topic
CRISPR-Associated ProteinsMajor topic
EndonucleasesMajor topic
RNA, Guide, CRISPR-Cas SystemsMajor topic
Base Sequence
DNA
Humans
Molecular Sequence Data

Source PubMed

Grants4

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated bySource
DP1 GM105378NIGMS NIH HHSUnited StatesDP1GM105378PubMed and NIHNIH RePORTER
P50 HG005550NHGRI NIH HHSUnited StatesP50HG005550PubMed and NIHNIH RePORTER
R01 GM088040NIGMS NIH HHSUnited StatesR01GM088040PubMed and NIHNIH RePORTER
GM105378NIGMS NIH HHSUnited StatesDP1GM105378PubMed and NIHNIH RePORTER

Trials0

The trials whose ClinicalTrials.gov record lists the paper, and the trial numbers the paper's PubMed record names.

None in this release.

Comments and corrections0

PubMed's comments and corrections, NLM's own indexing: retractions first, then expressions of concern, errata and updates, and comments last.

None in this release.

References30

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
123,934,178High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.Nat Biotechnol2013https://conductscience.org/library/papers/23934178PubMed
219,261,174Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.Genome Biol2009https://conductscience.org/library/papers/19261174PubMed
324,336,571Genome-scale CRISPR-Cas9 knockout screening in human cells.Science2014https://conductscience.org/library/papers/24336571PubMed
423,873,081DNA targeting specificity of RNA-guided Cas9 nucleases.Nat Biotechnol2013https://conductscience.org/library/papers/23873081PubMed
522,522,391Precision genome engineering with programmable DNA-nicking enzymes.Genome Res2012https://conductscience.org/library/papers/22522391PubMed
624,076,990Cas9 as a versatile tool for engineering biology.Nat Methods2013https://conductscience.org/library/papers/24076990PubMed
723,874,735Heritable and precise zebrafish genome editing using a CRISPR-Cas system.PLoS One2013https://conductscience.org/library/papers/23874735PubMed
823,945,932In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites.Nucleic Acids Res2013https://conductscience.org/library/papers/23945932PubMed
923,849,981CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.Cell2013https://conductscience.org/library/papers/23849981PubMed
1017,526,515Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool.Nucleic Acids Res2007https://conductscience.org/library/papers/17526515PubMed

1 to 10 of 30

See all 30 references

Cited by1,059

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 1,526 citations.

PMIDTitleJournalYearIn PapersSource
42,909,932Recent advances in CRISPR technologies for biomedical applications and disease treatment.Theranostics2026https://conductscience.org/library/papers/42909932PubMed
42,909,021Variable alterations in frequencies of CRISPR/Cas9-induced nucleotide deletion and insertion in transgenic maize callus tissues exposed to chromatin modifiers.Front Genome Ed2026https://conductscience.org/library/papers/42909021PubMed
42,824,655CRISPR/Cas9 in soybean research: a measured leap toward genetic refinement.Front Genome Ed2026https://conductscience.org/library/papers/42824655PubMed
42,805,266Ecology and evolutionary dynamics of the A-strain maize streak virus and the decade-long strategies targeting streak-resistant maize.Virus Res2026https://conductscience.org/library/papers/42805266PubMed
42,800,825Rational selection of SpCas9 and variant-derived adenine base editors for human genome editing.Gene Ther2026https://conductscience.org/library/papers/42800825PubMed
42,766,154Technical limitations of CRISPR-Cas9 genome editing in bacteria: challenges and future directions.Arch Microbiol2026https://conductscience.org/library/papers/42766154PubMed
42,758,354CRISPR-Cas9 genome editing: technological advances, delivery strategies and precision engineering of primary cells for therapeutic application in genetic disorders and diabetes mellitus.Mol Biol Rep2026https://conductscience.org/library/papers/42758354PubMed
42,754,914Species-dependent activities of the PINK1-parkin axis.Transl Neurodegener2026https://conductscience.org/library/papers/42754914PubMed
42,736,446HIV cure: from early therapies to cutting-edge strategies.J Neurovirol2026https://conductscience.org/library/papers/42736446PubMed
42,712,921High-Content CRISPR Screening: Methods and Applications.MedComm (2020)2026https://conductscience.org/library/papers/42712921PubMed

1 to 10 of 1,059

See all 1,059 cited by

Genes6

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

GeneNCBI Gene IDLinked byIn GenesSource
CLTA1,211PubTator's text mininghttps://conductscience.org/library/genes/1211NCBI Gene
CTRL1,506PubTator's text mininghttps://conductscience.org/library/genes/1506NCBI Gene
EMX12,016PubTator's text mininghttps://conductscience.org/library/genes/2016NCBI Gene
MFAP14,236PubTator's text mininghttps://conductscience.org/library/genes/4236NCBI Gene
RGN9,104PubTator's text mininghttps://conductscience.org/library/genes/9104NCBI Gene
VEGFA7,422PubTator's text mininghttps://conductscience.org/library/genes/7422NCBI Gene

Drugs0

The drugs whose trials, in the Pipelines release, list the paper.

None in this release.

Data1

The data bank entries PubMed's record names. Trial numbers are under Trials.

Data bankAccession
SRASRP033215

Source SRA

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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