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Paper28805804

A genetically encoded tool for manipulation of NADP+/NADPH in living cells.

Cracan V and 4 others. Nat Chem Biol, 2017. Cited by 110 papers, by iCite.

11 sourcespapers-2026-11-12-0.1.0

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ConductScience Library. A genetically encoded tool for manipulation of NADP+/NADPH in living cells. Papers, record 28805804. Release papers-2026-11-12-0.1.0, 12 Nov 2026. https://conductscience.org/library/papers/28805804

Paper

PubMed's record of the paper, with iCite's citation figures. No abstract is shown.

Title
A genetically encoded tool for manipulation of NADP+/NADPH in living cells.
PubMed
Journal
Nat Chem Biol
PubMed
Year
2017
PubMed
Publication types
Journal Article
PubMed
Citations, by iCite
110
iCite
Relative citation ratio, by iCite
3.37
iCite
Revised in PubMed
2026-11-02
PubMed

Authors5

The authors in the order PubMed's record lists them, with the ORCID iDs it states.

OrderAuthorForenameKindORCID iD
1Cracan VValentin
2Titov DVDenis Vhttps://orcid.org/0000-0001-5677-0651
3Shen HHongying
4Grabarek ZZenon
5Mootha VKVamsi K

Source PubMed

Topics6

NLM's MeSH indexing of the paper, its major topics first.

MeSH termMajor
NADH, NADPH OxidoreductasesMajor topic
NADPMajor topic
Protein EngineeringMajor topic
HeLa Cells
Humans
Oxidation-Reduction

Source PubMed

Grants8

Each grant PubMed's record writes, matched to the NIH project NIH's grant and paper links name for this paper, and each of those links the record does not write.

Grant, as the record writes itAgencyCountryNIH projectStated bySource
R01 GM099683NIGMS NIH HHSUnited StatesR01GM099683PubMed and NIHNIH RePORTER
K99 GM121856NIGMS NIH HHSUnited StatesK99GM121856PubMed and NIHNIH RePORTER
R00 GM121856NIGMS NIH HHSUnited StatesK99GM121856PubMed and NIHNIH RePORTER
R35 GM122455NIGMS NIH HHSUnited StatesR35GM122455PubMed and NIHNIH RePORTER
Howard Hughes Medical InstituteUnited StatesPubMedPubMed
S10OD016232NIHNIH RePORTER
S10OD021505NIHNIH RePORTER
U54DK110858NIHNIH RePORTER

References51

The papers the reference list in PubMed's record names, those in our copy of PubMed, in the list's order.

OrderPMIDTitleJournalYearIn PapersSource
12,585,516Substrate binding and catalysis by glutathione reductase as derived from refined enzyme: substrate crystal structures at 2 A resolution.J Mol Biol1989https://conductscience.org/library/papers/2585516PubMed
2789,350Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.J Bacteriol1976https://conductscience.org/library/papers/789350PubMed
327,124,460Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio.Science2016https://conductscience.org/library/papers/27124460PubMed
424,805,240Quantitative flux analysis reveals folate-dependent NADPH production.Nature2014https://conductscience.org/library/papers/24805240PubMed
52,540,822Switching kinetic mechanism and putative proton donor by directed mutagenesis of glutathione reductase.Biochemistry1989https://conductscience.org/library/papers/2540822PubMed
62,720,061Nicotinamide adenine dinucleotide fluorescence spectroscopy and imaging of isolated cardiac myocytes.Biophys J1989https://conductscience.org/library/papers/2720061PubMed
74,291,787The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver.Biochem J1967https://conductscience.org/library/papers/4291787PubMed
88,524,825A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.Proc Natl Acad Sci U S A1995https://conductscience.org/library/papers/8524825PubMed
921,982,714Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor.Cell Metab2011https://conductscience.org/library/papers/21982714PubMed
1017,076,895CellProfiler: image analysis software for identifying and quantifying cell phenotypes.Genome Biol2006https://conductscience.org/library/papers/17076895PubMed
112,924,777cDNA sequence of adrenodoxin reductase. Identification of NADP-binding sites in oxidoreductases.Eur J Biochem1989https://conductscience.org/library/papers/2924777PubMed
1218,399,646Pyridine nucleotide complexes with Bacillus anthracis coenzyme A-disulfide reductase: a structural analysis of dual NAD(P)H specificity.Biochemistry2008https://conductscience.org/library/papers/18399646PubMed
1327,648,601A General Tool for Engineering the NAD/NADP Cofactor Preference of Oxidoreductases.ACS Synth Biol2017https://conductscience.org/library/papers/27648601PubMed
1426,999,781Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.Nat Chem Biol2016https://conductscience.org/library/papers/26999781PubMed
1517,295,611The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.Biochem J2007https://conductscience.org/library/papers/17295611PubMed
167,650,022Feasibility of a mitochondrial pyruvate malate shuttle in pancreatic islets. Further implication of cytosolic NADPH in insulin secretion.J Biol Chem1995https://conductscience.org/library/papers/7650022PubMed
176,822,532The catalytic mechanism of glutathione reductase as derived from x-ray diffraction analyses of reaction intermediates.J Biol Chem1983https://conductscience.org/library/papers/6822532PubMed
183,663,177Redox state and lactate accumulation in human skeletal muscle during dynamic exercise.Biochem J1987https://conductscience.org/library/papers/3663177PubMed
1927,049,945Reductive carboxylation supports redox homeostasis during anchorage-independent growth.Nature2016https://conductscience.org/library/papers/27049945PubMed
2024,757,503Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD(P)(+) Regeneration in Biocatalytic Oxidations.Comput Struct Biotechnol J2014https://conductscience.org/library/papers/24757503PubMed

See all 51 references

Cited by74

The papers in our copy of PubMed whose reference list names this one, newest first. iCite, which reads more sources, counts 110 citations.

PMIDTitleJournalYearIn PapersSource
42,934,322Enzymatic oxidative esterification with an unconventional regioselective control.Nat Chem2026https://conductscience.org/library/papers/42934322PubMed
42,875,582Genetic modulation of NAD(P)H homeostasis reveals distinct redox-coupled metabolic vulnerabilities.Nat Metab2026https://conductscience.org/library/papers/42875582PubMed
42,719,331Metabolic circuitry of NADH buffering reveals a vulnerability in redox-adaptive cancer cells.Innovation (Camb)2026https://conductscience.org/library/papers/42719331PubMed
42,711,485Increasing cytosolic NADPH to mimic exercise.Nat Metab2026https://conductscience.org/library/papers/42711485PubMed
42,711,484Pentose phosphate pathway-derived NADPH facilitates physiological hypertrophy and alleviates ischemia-reperfusion injury in the heart.Nat Metab2026https://conductscience.org/library/papers/42711484PubMed
42,645,669The Reductive Stress Nexus: Integrated Pathways of Metabolic Dysregulation, Senescence and Blood-Brain Barrier Failure in Neurodegenerative Disease and Brain Aging.Cell Biochem Biophys2026https://conductscience.org/library/papers/42645669PubMed
42,229,415Reductive death is averted by a conserved de novo lipogenic switch.Mol Cell2026https://conductscience.org/library/papers/42229415PubMed
42,062,483The atypical E3 ligase HOIL-1 safeguards the ribosome during cellular stress.Nat Cell Biol2026https://conductscience.org/library/papers/42062483PubMed
41,872,217Mitochondrial metabolic imbalance drives diploidization in mouse haploid embryonic stem cells via NADPH overload.Nat Commun2026https://conductscience.org/library/papers/41872217PubMed
41,842,958The allosteric IDH1 inhibitor ivosidenib overcomes chemoresistance in intrahepatic cholangiocarcinoma models expressing wild-type IDH1.J Clin Invest2026https://conductscience.org/library/papers/41842958PubMed
41,717,224Changes in the brain [NAD+]/[NADH] and [NADPH]/[NADP+] with aging and anti-aging dietary restriction.Front Aging Neurosci2026https://conductscience.org/library/papers/41717224PubMed
41,703,108DHODH turns cytosolic, yet cells keep growing.Nat Metab2026https://conductscience.org/library/papers/41703108PubMed
41,699,300A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicity.Nat Metab2026https://conductscience.org/library/papers/41699300PubMed
41,549,107Modeling tissue-specific Drosophila metabolism identifies high sugar diet-induced metabolic dysregulation in muscle at reaction and pathway levels.Nat Commun2026https://conductscience.org/library/papers/41549107PubMed
41,481,756Perturbation of NAD(P)H metabolism with the LbNOX xenotopic tool extends lifespan and mitigates age-related changes.Sci Adv2026https://conductscience.org/library/papers/41481756PubMed
41,337,482Oxidative pentose phosphate pathway is required for T cell activation and antitumor immunity.Proc Natl Acad Sci U S A2025https://conductscience.org/library/papers/41337482PubMed
41,252,749Genetically Reprogramming Crops and Rhizobacteria for Nutritional Iron Biofortification.ACS Synth Biol2025https://conductscience.org/library/papers/41252749PubMed
41,226,410Potassium-Hydroxide-Based Extraction of Nicotinamide Adenine Dinucleotides from Biological Samples Offers Accurate Assessment of Intracellular Redox Status.Int J Mol Sci2025https://conductscience.org/library/papers/41226410PubMed
41,184,229Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging.Nat Commun2025https://conductscience.org/library/papers/41184229PubMed
40,446,642PRPS activity tunes redox homeostasis in Myc-driven lymphoma.Redox Biol2025https://conductscience.org/library/papers/40446642PubMed

See all 74 cited by

Genes9

The genes the Gene search release ties to the paper: Swiss-Prot's references first, then PubTator's text mining.

GeneNCBI Gene IDLinked byIn GenesSource
ATP5PF522PubTator's text mininghttps://conductscience.org/library/genes/522NCBI Gene
DECR11,666PubTator's text mininghttps://conductscience.org/library/genes/1666NCBI Gene
G6PD2,539PubTator's text mininghttps://conductscience.org/library/genes/2539NCBI Gene
GSR2,936PubTator's text mininghttps://conductscience.org/library/genes/2936NCBI Gene
LRPPRC10,128PubTator's text mininghttps://conductscience.org/library/genes/10128NCBI Gene
OGDH4,967PubTator's text mininghttps://conductscience.org/library/genes/4967NCBI Gene
PHGDH26,227PubTator's text mininghttps://conductscience.org/library/genes/26227NCBI Gene
POTEF728,378PubTator's text mininghttps://conductscience.org/library/genes/728378NCBI Gene
TET3200,424PubTator's text mininghttps://conductscience.org/library/genes/200424NCBI Gene

Data dates

The date of each source this release read.

PubMed
2026-11-06
PubMed
iCite
2026-11-05
iCite
PMC's ID file
2026-11-05
PMC's ID file
NIH RePORTER's grant and paper links
2026-11-01
NIH RePORTER's grant and paper links
Trials
2026-11-12
Gene search
2026-11-11
Pipelines
2026-09-18

None in this release

  • Trials
  • Comments and corrections
  • Drugs
  • Data

Sources 11

  • ChEMBL, through Open TargetsCC BY-SA 3.0
  • ClinicalTrials.govNo license stated
  • HGNCCC0
  • iCiteCC BY 4.0
  • NCBI GenePublic domain (US government)
  • NIH RePORTER's grant and paper linksPublic domain
  • Open TargetsCC0 1.0
  • PMC's ID filePublic domain (US government)
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0
Each source's terms
  • ChEMBL, through Open TargetsCC BY-SA 3.0, for the parts from ChEMBL. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • ClinicalTrials.govnone stated
  • HGNCCC0: https://www.genenames.org/about/license/
  • iCiteCC BY 4.0
  • NCBI GeneNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • NIH RePORTER's grant and paper linksPublic domain (NIH RePORTER)
  • Open TargetsCC0 1.0. Open Targets asks that users cite its latest paper: Buniello et al. 2025, Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery, Nucleic Acids Research 53(D1):D1467, PMID 39657122. Read on 8 Nov 2026 at https://platform-docs.opentargets.org/licence
  • PMC's ID fileNone named for the file. NCBI says information created by or for the US government on its site is in the public domain, and asks that NLM be acknowledged: https://www.ncbi.nlm.nih.gov/home/about/policies/
  • PubMedNLM's terms
  • PubTator 3Public domain
  • UniProtCC BY 4.0. UniProt's licence file: "All databases and documents in the UniProt FTP directory may be copied and redistributed freely, without advance permission, provided that this copyright statement is reproduced with each copy." UniProt asks that users cite its latest paper: The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2025, Nucleic Acids Research 53:D609-D617, https://doi.org/10.1093/nar/gkae1010. Read on 9 Nov 2026 at https://www.uniprot.org/help/license

ClinicalTrials.gov's data carry an international copyright outside the United States: https://clinicaltrials.gov/about-site/terms-conditions.

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